Isolation and Activity Test of Acidophilic Iron and Sulfur Oxidizing Bacteria from Black Water Ecosystem of Central Kalimantan

Abstract

The acidophilic iron and sulfur oxidizing bacteria were isolatedfrom black water ecosystem, an 'extreme' ecosystem affected indirect or directly by peat land Isolation and selection were done on minimal media (liquid and solid). All selected strain of bacteria (BB 179, OM 349, AH 41, TB 23, TB 27, TP 3, NN 323, and SI 188) were identified as Thiohacillus ferrooxidans. Biooxidation and bio-leaching tests were accomplished using the isolated bacteria. The results showed the capability of the isolated bacteria to oxidize ferrous-salt and to leach the low qualities of sulfide ores.