The Selection of Xylanase-Producing Indegenous Bacteria

Abstract

The isolation and selection of xylanase producing bacteria have been done from soil and waste of starch Industry. Strains were cultivated in neutral and alkaline media with xilan as a substrate. Colonies which produced clearing zone were presumed as xylanolytic bacteria and chosen for further screening. Results show that 10 isolates of bacteria In neutral condition and 25 bacteria in alkaline condition. There are three isolates in neutral condition ON. 12, ON-13 and ON-33 have high enzyme activities at 19.93, 23.42 and 19.32 U/ml, and specific activities which were 80.37, 71.85 and 114.36 U/mg protein respectively. On the other hand there are two isolates in alkaline condition TA IS and TA-fl15 which have high enzyme activities at 12.43 and 11.18 U/mI, and specific activities which were 36.72 and 42.68 U/mg protein respectively.