Hydrolysis Methods of Starch for Culture Media of Bacillus sp. BMN14 Producing Biosurfactant Lipopeptide

Abstract

Ezymatic and acid hydrolysis of starch for culture media of Bacillus sp. BMN14 producing lipopeptide blosurfactant were studied using cassava, arrowroot and sago starch. These starch bydrolysates were used as glukose substitute In the were to culture media of biosurfactant.producing bacteria. Starch hydrolyzed from arrowroot 73.08-83.5% and cassava 80.0-87.98% yielded glucose higher than sago 62.5-64.5%. Capability of the bacteria to produce biosurfactant represented as the amount of acid precipitate 0.78 g/l and surface tension of the broth 33.6 mN/rn in the culture medium contained enzymatic hydrolyzed-starch.