isolation and Selection of Extremophillic Cellulase Producing Bacteria Isolatedfrom Black Water Ecosystem

Abstract

The cellulolytic bacteria are potential organisms for biotechnological applications such as in decomposition of lignocellulose materials and enzyme technology. The black water ecosystem is a unique ecosystem because of its high diversity of flora, fauna, and microbes. The aims of this experiment were to isolate cellulolytic bacteria from the ecosystem of black water and to select their ability to grow at the extrem conditions low and high plls and high temperature. The total number of cellulolytic bacteria isolated at 30°C with pH 4 or 7 and at 50°C with pH 4 or 7 was 1.263. In further screening at higher temperatures, 30 bacteria were obtained at 60°C while at 70°C only 16 bacteria were selected. The isolates were furthter screened at different pH based on their optimal growth temperature, and they were able to grow at pH 3-11. Besides bacteria that grow at normal temperature 4-40°C and at p11 neutral 5.8.5 there at high temperatures 60 and 70°C and low and high pils 3 and 10, 11 showing that there are high diversity in the ecosystem of black water.