Lalat penggorok daun Liriomyza spp. (Diptera: Agromyzidae) dan parasitoidnya pada sayuran dataran tinggi dengan perhatian utama pada parasitoid Opius chromatomyiae Belokobylskij & Wharton(Hymenoptera: Braconidae)

Abstract

Leafminers Liriomyza spp. are major pest on highland vegetables in Indonesia. The objectives of the research were to (1) evaluate leafminer and its parasitoids on higland vegetables, (2) study the intensity of parasitism of O. chromatomyiae in various host plants, (3) study the population dynamic of leafminer and its parasitoid in shallot, (4) evaluate the impact of three insecticides on parasitoid O. chromatomyiae, and (5) evaluate the demographic parameters and functional response of parasitoid O. chromatomyiae. Surveys of leafminer population and its parasitoid were undertaken by collecting 20-40 infested leaves. Leafminer flies and parasitoids emerged were collected and identified. Level of parasitism was calculated based on the number of parasitoids emerged divided by the total number of flies and wasps emerged. The population dynamic of leafminer and its parasitoids were studied on two varieties of green onion, by collecting 20 leaves and counting number of flies catched on yellow traps every week. The impact of insecticides on both leafminers and parasitoids was conducted by applying three different insecticides: abamectin, azadirachtin, and cartaphydrochloride once a week at the recommended dosage. Life table of O. chromatomyiae was studied to determine the population growth potential of the parasitoid, by calculating reproduction rate, intrinsic rate of increase, generation time, and stable age distribution. Functional response was evaluated by exposing O. chromatomyiae on the third instar larvae of L. huidobrensis at eight different host densities (2, 4, 8, 10, 12, 16, 20, and 24) of. Our surveys revealed that Liriomyza huidobrensis, L. sativae, L. chinensis and Chromatomyia horticola were associated with various highland vegetables. Eight parasitoids emerged from leafminer-infested leaves: Hemiptarsenus varicornis, Asecodes deluchii, Neochrysocharis okazakii, N. formosa, Neocrhysocharis sp. Quadrastitchus liriomyzae, O. chromatomyiae and Gronotoma micromorpha. Parasitoid Opius chromatomyiae emerged from 13 different species of vegetables infested by leafminers.. Number of leafminers and parasitoids emerged, and crop damage were significantly different between two green onion varieties tested. Cartap hydrochloride was the most effective insecticide in term for reducing leafminer populations. Even though not as effective as cartap hydrochloride, abamectin and azadirachtin could reduce level of leafminer damage and had lower detrimental effects on natural enemies. Life table studies showed that longevity of females Opius chromatomyiae (9.95 days) shorter than males (11.73 days). Females laid 104.73 eggs with the oviposition rate was 9.31 eggs/days. Reproductive output (R0) was 28.55 with generation time (T) was about 15.96 days and intrinsic rate of increase was 0.21. The stable age ditribution was 37.93% eggs, 24.92% larvae, 20.36% pupae and 16.78% adults. Functional response of O. chromatomyiae is type II (hyperbolic curve) with searching time was 0.0777/hour and handling time was 2.5818/hour.