Bioekologi dan deteksi senyawa bioaktif rotifera Brachionus spp. dari perairan pantai dan estuari Sulawesi Utara

Abstract

One of the most important microorganism s is the plankton. The existence of plankton affect the water where they live because this organism has an important role as source of food for many marine organisms. Rotifers are microscopic aquatic animals of the phylum Rotifera. The research aims to find out the existence and the most dominant rotifers in coastal territorial waters and estuaries in North Sulawesi, and the relations hip between their abundance and some environmental parameters. To compare morphometry of wild rotifers Brachionus rotundiformis from different locations a nd the cultured rotifers fed with different microalgae within different salinities, analysing the life cycle and detecting bioactive compound. The research was done in coastal ar eas and estuaries of North Sulawesi. Plankton sampling was done in several br ackishwater ponds and coastal areas along Manembo-nembo, Minanga, Wori , and Tumpaan. Samplings were conducted during east and west seas ons, as well as high and low tides. Temperature, salinity, pH, turbidity, and dissolved oxygen were measured in situ using test kit water. Samples of rotifer s were collected by deploying a pankton net horizontally along 10 m, and were pres erved using formaldehyde solution for further identification and measurements. Th e rotifers were cultured with different salinity (4, 20, 40, 50, 60 ppt) and fe d with different micro algae ( Nannochloropsis oculata and Prochloron species). The measurement for morphology characteristic were conducted on lorica length, lorica width and anterior width.