Perbanyakan In Vitro Tanaman Bawang Putih (Allium sativum L.) Varietas Lumbu Putih Melalui Induksi Tunas Adventif

Abstract

Two sets of' experiments were conducted to determine the effect of growth hormones, argynine. and coconut water, on the adventitious shoot induction from garlic tissue, and also to find out the besr medium fbr udventitious shoots proliferation. Both experiments could induce direct adventitious shoot and indirect adventitious shoot formation . fiom calli. Medium with 2 ppm Kinetin and 0.4 pprn 2,4-D produce good quantity and quality of shoots. The number of' shoot fiom this medium were 32.6 shoots per explant. Medium with 0.5 ppm Kinetin, 0.1 pprn 2,4-0, 25 ppm Argynine, and 10% coconut water produce the highest diameter and good quality 01 calli, while medium with I pprn 2iP and 25 pprn Argynine induced adventitious shoot@om calli and produced the highest number of shoot per cuiture (33.9 shoots).