Cloning, Homological Analysis, and Expression of DNA Pol I from Geobacillus thermoleovorans

Abstract

DNA POLI gene from Geobacillus thermoleovorans (DNA POLI ITB) was cloned and overexpressed in Escherichia coli using two steps PCR aplification. The gene was isolated and sequenced to consist of 2628 nucleotides that encode 876 amino acids.