In Vitro Plantlet Production And Field Establishment Asparagus Officinalis

Abstract

Shoot multiplication and plantlet formation of Asparagus officinalis L. was studied in order to support large scale plantation in Indonesia.. Cultures were grown in MS media supplemented with 0.5 mg/l 2-iP. Auxin : IAA, IBA, and NAA at the rate of 0.25, and 0.5 mg/l, were used. Number of shoots formed reaching an average of 22.8 was obtained in cultures established in 2-iP 0.50 mg/l and IAA 0.50 mg/l, while those in 2-iP and NAA showed the lowest number of shoots. However 92.3 % of the cultures in NAA medium formed roots. Only 10 % of the cultures in NAA medium formed roots. Upon transferring to the soil, 82 % of the plantlets from NAA media survived and showed a normal appearance. After 4 months in the soil, the plantlets reached an average length of 40 cms.