Isolation and characterization of polymorphic microsatellite markers in Amorphophallus paeoniifolius (Dennst.) Nicolson, Araceae

Abstract

Amorphophallus paeoniifolius is an herbaceous perennial tuber crop distributed widely in many Asian countries. We isolated 19 polymorphic loci from A. paeoniifolius using a dual-suppression-PCR technique. These loci provide microsatellite markers with high polymorphism ranging from three to 24 alleles per locus. The observed and expected heterozygosities ranged from 0.521 to 0.854 and from 0.766 to 0.930, respectively. This high allelic diversity indicates that our markers are suitable for a population study in A. paeoniifolius. Keywords Araceae • elephant foot yam • microsatellites • tuber crop