Isolasi, Identifikasi, dan Karakterisasi Alkaloid dari Kulit Johar (Cassia siamea Lamk.)

Abstract

This purpose of this research is to idate, to identify and to chmtirlze the content of alkaloid of the bark of Cassia sigmea Lamk. The bark of Cassia is used traditionally for curing the disturbancy of stomach and purgative agent. Supposing that bark of Cassia contains alkaloids. The alkaloid from the issolation makes orange precipitation with Dragendorf reagent, white-yellow precipication with Meyer reagent and chocelate precipitation with Wagner reagent. Thin layer chromatography method to this alkaloid gives orange spots with the Dragendorf reagent, yellow with iod vapor, and green-yellow with sulfuric acid 10 %. The ultraviolet absorption of this alkaloid has maximum absorption at the wave length of 225.0 nm, 252.0 nm, and 337.5 urn. The infrared absorption shows the content of the OH, C = 0, C - N, N - H, C - H, and aromatic groups. From this ultraviolet absorption shows that this alkaloid has rings of benzena.