Eksplorasi, Isolasi, dan Karakterisasi Bakteri Dekomposer dalam Pengembangan Bioaktivator Kompos

Abstract

Environmental Technology and Security (ETS) section SEAMEO BIOTROP pada tahun 2023 menghasilkan BIOTROP Kompos (BIOPOS) sebagai hasil pengelolaan mandiri sampah dedaunan dan baglog jamur di SEAMEO BIOTROP. Namun, BIOPOS masih perlu dikembangkan lebih lanjut untuk dilakukan eksplorasi bakteri dekomposer yang dapat dijadikan bioaktivator BIOPOS guna mempercepat proses pengomposan dan meningkatkan kualitas kompos yang dihasilkan. Penelitian ini bertujuan mengeskplorasi bakteri dekomposer untuk pengembangan bioaktivator BIOPOS, mengisolasi bakteri dekomposer untuk pengembangan bioaktivator BIOPOS, karakterisasi bakteri dekomposer untuk pengembangan bioaktivator BIOPOS, dan menguji efektivitas bakteri dekomposer dalam mendekomposisi bahan BIOPOS. Isolat bakteri BIOPB, BIOKB, BIOPI, KSCMI, dan TJCMB di karakterisasi secara morfologi, fisiologi, dan biokimia. Metode eksperimental dilakukan dalam pembuatan dan pengaplikasian bioaktivator BIOPOS. Hasil karakterisasi menunjukkan isolat bakteri BIOPB, BIOKB, KSCMI, dan TJCMB berpotensi sebagai bakteri dekomposer. Isolat bakteri dekomposer lebih efektif dalam mendekomposisi bahan BIOPOS dibandingkan EM4 dilihat dari tingginya suhu BIOPOS yang mencapai 52°C.

The Environmental Technology and Security (ETS) section of SEAMEO BIOTROP 2023 produced BIOTROP Compost (BIOPOS) as a result of self management of leaf waste and mushroom baglogs at SEAMEO BIOTROP. However, BIOPOS still needs further development to explore decomposer bacteria that can be used as BIOPOS bioactivators to accelerate the composting process and improve the quality of the compost produced. This study aims to explore decomposer bacteria for the development of BIOPOS bioactivators, isolate decomposer bacteria for the development of BIOPOS bioactivators, characterize decomposer bacteria for the development of BIOPOS bioactivators, and test the effectiveness of decomposer bacteria in decomposing BIOPOS materials. Bacterial isolates BIOPB, BIOKB, BIOPI, KSCMI, and TJCMB were characterized morphologically, physiologically, and biochemically. Experimental methods were used to produce and apply the BIOPOS bioactivator. The characterization results showed that the bacterial isolates BIOPB, BIOKB, KSCMI, and TJCMB have potential as decomposer bacteria. The decomposer bacterial isolates were more effective in decomposing BIOPOS materials than EM4, as seen from the high temperature of BIOPOS which reached 52°C.