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      Studi Kasus: Perbandingan Metode Analisis Tanin untuk Mengatasi Krisis Pengadaan Bahan Indigo Karmin

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      Date
      2024
      Author
      Mutiara, Ayu
      Sarastani, Dewi
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      Abstract
      Tanin merupakan senyawa pada teh yang dapat digunakan sebagai pedoman mutu karena memberikan cita rasa yang khas terhadap teh tersebut yaitu rasa yang sedikit sepat. Metode yang biasa digunakan untuk menganalisis kadar tanin yaitu dengan metode titrasi peranganometri. Namun, terdapat kendala dalam pengadaan bahan reagen indigo karmin. Penelitian ini bertujuan untuk mengetahui efektivitas perbandingan hasil analisis kadar tanin menggunakan metode titrasi permanganometri dan spektrofotometri. Berdasarkan hasil uji-t yang diperoleh nilai t stat sampel bebas independent yang dihasilkan t stat (thitung) haitu -1,67446, dengan nilai t critical two tailed (ttabel) sebesar 2,17881, dan nilai P(T<=t) two tailed (p value) sebesar 0,020144. Hal ini menunjukan bahwa metode spektrofotometri dan tanin pada analisis tanin berbeda nyata pada tingkat signifikansi 5%, karena nilai P(T<=t) two tailed (p value) < a 0,05 sehingga H0 diterima. Faktor penyebab terjadinya penurunan kadar tanin metode spektrofotometri bisa disebabkan oleh suhu dan hidrolisis.
       
      Tannin is a compound in tea that can be used as a quality guide because it gives the tea a distinctive taste, namely a slightly astringent taste. The method commonly used to analyze tannin levels is the permanganometric titration method. However, there are obstacles in procuring the indigo carmine reagent. This research aims to determine the effectiveness of comparing the results of tannin content analysis using the permanganometric and spectrophotometric titration methods. Based on the results of the t-test, the t stat value obtained for the independent free sample resulted in a t stat (tcount) of -1.67446, with a critical two-tailed t value (ttable) of 2.17881, and a P(T<=t) value of two-tailed (p value) is 0.020144. This shows that the spectrophotometric and tannin methods in tannin analysis are significantly different at the 5% significance level because the two-tailed P(T<=t) value (p-value) < a 0.05 so H0 is accepted. Factors causing a decrease in tannin levels using spectrophotometric methods can be caused by temperature and hydrolysis.
       
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      http://repository.ipb.ac.id/handle/123456789/157244
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