Resistansi Isolat Escherichia coli pada Daging Soto Ayam yang Dijual di Lingkar Kampus Dramaga IPB University

Abstract

Makanan khususnya yang dijual di pinggir jalan rentan terkontaminasi oleh Escherichia coli (E. coli) salah satunya adalah soto ayam. Escherichia coli patogen sering kali bersifat resistan terhadap antibiotik yang mengakibatkan bahaya bagi manusia karena infeksi yang semula mudah diobati dengan antibiotik kini menjadi kurang efektif. Tujuan penelitian ini yaitu mengidentifikasi resistansi antibiotik dari bakteri E. coli yang diisolasi dari daging ayam suwir soto ayam yang dijual di lingkar Kampus IPB, Dramaga, Bogor. Sampel yang digunakan sebanyak 21 isolat yang telah diuji positif terhadap keberadaan E. coli. Pengujian resistansi antibiotik menggunakan metode Kirby-Bauer disk diffusion dan mengacu pada Clinical Laboratory Standard Institute 2023. Hasil penelitian menunjukkan bahwa 11 isolat E. coli mengalami resistansi terhadap ampisilin (33,33%), tetrasiklin (23,81%), sulfametoksazol-trimethoprim (14,29%), sefotaksim (19,05%), streptomisin (4,76%), dan terendah adalah antibiotik kloramfenikol (9,52%). Empat isolat E. coli bersifat multi-drug resistance dengan sebagian besar pola ampisilin-tetrasiklin- sulfametoksazol trimetoprim-sefotaksim. Keberadaan E. coli yang resistan antibiotik dapat menimbulkan ancaman kesehatan masyarakat.

Food especially those sold on the roadside, is susceptible to contamination by Escherichia coli (E. coli) one of them is chicken soto. Pathogenic Escherichia coli is often resistant to antibiotics which can cause danger to humans because infections that were previously easily treated with antibiotics are now less effective. The purpose of this study was to identify antibiotic resistance from E. coli isolated from shredded chicken in chicken soto sold around IPB University, Dramaga, Bogor. The samples used were 21 isolates that had been tested positive for the presence of E. coli. The antibiotic resistance was examined using the Kirby-Bauer disk diffusion method and referred to the Clinical Laboratory Standard Institute 2023. The results indicated that 11 E. coli isolates were resistant to ampicillin (33.33%), tetracycline (23.81%), sulfamethoxazole-trimethoprim (14.29%), cefotaxime (19.05%), streptomycin (4.76%), and the lowest was the antibiotic chloramphenicol (9.52%). Four E. coli isolates were multi-drug resistant, predominantly showing the pattern of ampicillin-tetracycline-sulfamethoxazole trimethoprim-cefotaxime. The ocurance of antibiotic resistance E. coli can pose a public health threat.