Identifikasi dan Karakterisasi Bakteri Asam Laktat asal Feses Burung Cenderawasih (Paradisaeidae sp.)

Abstract

Bakteri asam laktat (BAL) diisolasi menggunakan metode streak pada media de Man, Rogosa and Sharpe Agar (MRSA). Media MRSA mendukung pertumbuhan bakteri asam laktat termasuk genus Lactobacillus. Identifikasi koloni bakteri dari 4 sampel feses burung cenderawasih secara makroskopis, mikroskopis, dan uji biokimia. Koloni teridentifikasi sebagai bakteri asam laktat dilakukan karakterisasi probiotik mencakup uji ketahanan garam NaCl (NaCl 2%, 4%, dan 6,5%), uji ketahanan pH (pH 2 dan pH 3), serta uji ketahanan garam empedu (MRS Bile Salt Broth 0,3% dan 3%). Hasil penelitian menunjukkan bahwa dari 8 isolat yang diidentifikasi, 7 isolat BAL diuji untuk karakterisasi sebagai kandidat probiotik. Hasil karakterisasi pada 7 isolat BAL menunjukkan 5 bakteri memiliki karakter sebagai kandidat probiotik yakni Aerococcus sp., Pediococcus sp., Streptococcus sp., Leuconostoc sp., dan Weisella sp. Penelitian ini memberikan informasi tentang bakteri asam laktat sebagai kandidat probiotik pada saluran pencernaan burung cenderawasih demi mengoptimalkan proses pencernaan, sehingga dapat meningkatkan kesehatan saluran pencernaan burung cenderawasih.

Lactic acid bacteria (LAB) were isolated using the streak method on de Man, Rogosa and Sharpe Agar (MRSA) media. MRSA media supports the growth of lactic acid bacteria including the genus Lactobacillus. Identification of bacterial colonies from 4 bird of paradise feces samples using macroscopic, microscopic and biochemical tests. Colonies identified as lactic acid bacteria were subjected to probiotic characterization including a NaCl salt resistance test (2%, 4% and 6.5% NaCl), a pH resistance test (pH 2 and pH 3), and a bile salt resistance test (MRS Bile Salt Broth 0.3 % and 3%). The results showed that of the 8 isolates identified, 7 LAB isolates were tested for characterization as probiotic candidates. The characterization results of 7 LAB isolates showed that 5 bacteria had characteristics as probiotic candidates, namely Aerococcus sp., Pediococcus sp., Streptococcus sp., Leuconostoc sp., and Weisella sp. This research provides information about lactic acid bacteria as probiotic candidates in the digestive tract of birds of paradise in order to optimize the digestive process, thereby improving the health of the digestive tract of birds of paradise.