Histopatologi Ginjal Tikus yang Diinduksi DMBA untuk Pemodelan Tumor pada Kulit dan Diobati Emulsi Nanopartikel Ekstrak Keladi Tikus

Abstract

Penelitian ini bertujuan mengevaluasi potensi efek toksisitas bahan herbal antitumor emulsi nanopartikel ekstrak keladi tikus pada histopatologi ginjal tikus putih yang diinduksi karsinogen 7.12-dimethylbenz(a)anthracene (DMBA). Sebanyak dua puluh lima ekor tikus jantan berumur 30 hari dengan bobot badan 70-80 gram yang telah diinduksi DMBA digunakan dalam penelitian ini. Tikus dibagi menjadi lima kelompok perlakuan, yaitu kelompok kontrol negatif, kelompok perlakuan pelarut emulsi nanopartikel, kelompok perlakuan emulsi nanopartikel keladi tikus dosis rendah, kelompok perlakuan emulsi nanopartikel keladi tikus dosis sedang, dan kelompok perlakuan emulsi nanopartikel keladi tikus dosis tinggi. Pengamatan perubahan histopatologi ginjal dilakukan secara mikroskopik dengan pewarnaan Hematoksilin Eosin dan perbesaran 100x. Lesio histopatologis yang diamati dari masing-masing kelompok perlakuan berupa hemoragi, infiltrasi sel radang, dan nekrosis tubular. Analisis histopatologi dilakukan menggunakan aplikasi ImageJ dan data kuantitatif dianalisis dengan metode one-way analysis of variance (ANOVA). Hasil pengamatan seluruh kelompok perlakuan menunjukkan bahwa hemoragi, infiltrasi sel radang, dan nekrosis tubular secara signifikan tidak berbeda nyata (p

This study aims to evaluate the potential toxicity effect of the antitumor herbal ingredient of emulsion nanoparticles rodent tuber extract on kidney histopathology of white rats induced by the carcinogen 7.12-dimethylbenz(a)anthracene (DMBA). A total of twenty-five 30-day-old male mice with a body weight of 70-80 grams that had been induced by DMBA were used in this study. The mice were divided into five treatment groups, namely the negative control group, the nanoparticle emulsion solvent treatment group, the low dose rodent tuber nanoparticle emulsion treatment group, the medium dose rodent tuber nanoparticle emulsion treatment group, and the high dose rodent tuber nanoparticle emulsion treatment group. Observation of kidney histopathological changes was carried out microscopically with Hematoxylin Eosin staining and 100x magnification. Histopathological lesions observed in each treatment group were hemorrhage, inflammatory cell infiltration, and tubular necrosis. Histopathological analysis was carried out using the ImageJ application and quantitative data was analyzed using the one-way analysis of variance (ANOVA) method. Observation results for all treatment groups showed that hemorrhage, inflammatory cell infiltration and tubular necrosis were not significantly different (p>0.05). Based on the results, it was concluded that in this study treatment using rat taro was not effective in suppressing histopathological damage to rat kidneys due to the carcinogen DMBA. There was no visible toxic effect on the kidney histopathology of white rats at either low or high doses of rat taro extract nanoparticle emulsion