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      Potensi Cendawan Entomopatogen sebagai Antioksidan dan Antimikrob

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      Date
      2024
      Author
      Nurhabibah, Qori
      Listiyowati, Sri
      Astuti, Rika Indri
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      Abstract
      Beberapa senyawa bioaktif dalam cendawan entomopatogen dapat berperan sebagai antioksidan dan antimikrob. Hal ini mendorong dilakukannya penelitian terhadap potensi cendawan entomopatogen YSP19 dan YSP24 koleksi Laboratorium Mikologi-Departemen Biologi, FMIPA, IPB sebagai antioksidan alami dan antimikrob. Metode yang digunakan terdiri atas peremajaan cendawan sampel, pembuatan ekstrak dengan pelarut metanol 80%, pengukuran aktivitas antioksidan terhadap DPPH, serta uji antagonis cendawan sampel melalui dual culture terhadap Escherichia coli ATCC 8739, Staphylococcus aureus ATCC 6538, dan Ganoderma boninense IPBCC 10 0658. Cendawan sampel YSP19 dan YSP24 memiliki nilai aktivitas antioksidan yang lemah ditandai dengan nilai IC50 secara berturut sebesar 614,84 µg/mL dan 4420,01 µg/mL. Potensi antagonis cendawan terhadap kedua bakteri ditandai dengan zona bening setelah 24 jam masa inkubasi. Diameter zona bening paling besar terdapat pada cendawan YSP19 terhadap Escherichia coli ATCC 8739, yaitu 9,8 mm, sedangkan cendawan YSP24 terhadap Staphylococcus aureus ATCC 6538 menghasilkan diameter zona lebih kecil, yaitu 5,3 mm. Selain itu, cendawan YSP19 mampu menghambat Ganoderma boninense IPBCC 10 0658 hingga 67,78%, sedangkan cendawan YSP24 hanya mampu menghambat sebesar 46,67% pada hari ke-14 masa inkubasi.
       
      Several bioactive compounds in entomopathogenic fungi can act as antioxidants and antimicrobials. This study aimed to determine the potential of the entomopathogenic fungi YSP19 and YSP24 from the collection of the Mycology Laboratory, Department of Biology, FMIPA, IPB, as a source of antioxidants and antimicrobials. The method used consisted of rejuvenating the sample fungus, making an extract using 80% methanol solvent, measuring antioxidant activity against DPPH, and testing the antagonist of the sample fungus through dual culture against Escherichia coli ATCC 8739, Staphylococcus aureus ATCC 6538, and Ganoderma boninense IPBCC 10 0658. YSP19 and YSP24 fungus samples had weak antioxidant activity values, with IC50 values of 614,84 µg/mL and 4420,01 µg/mL, respectively. A clear zone indicated the potential antagonist of the fungus against both bacteria after 24 hours of incubation. The largest clear zone diameter found in the YSP19 fungus against Escherichia coli ATCC 8739 was 9,8 mm, while the YSP24 fungus produced a minor zone diameter of 5,3 mm against Staphylococcus aureus ATCC 6538. The YSP19 fungus inhibited Ganoderma boninense IPBCC 10 0658 up to 67,78%, while the YSP24 fungus could only inhibit 46,67% on the 14th day of the incubation period.
       
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      http://repository.ipb.ac.id/handle/123456789/153118
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      Indonesia DSpace Group 
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