Isolasi dan karakterisasi mikroorganisme asal sampah organik pasar dan isi rumen serta aplikasinya sebagai starter dalam pengomposan sampah

Abstract

Waste is a negative side of materials that can cause nuisance, putrid odor and can become a growth media for contagious desease. Waste is a problem for people and goverment, for example evironment polution. Organic waste handling by Bsing nature ability needs long time. Microorganisms addition as a starter in waste composting can be as alternative to solve time problem. Indigenous starter in pitrid wastes or rumen substance naturally have an ability to adapt with evironment and decompose wastes. Isolation microorganisms from wastes and rumen substance which have ability to decompose cellulose very interested to do, especially it's aplications in composting, to solve wastes problems in big city. Based on that reason, this research was done. Ability of microorganisms isolate as starter observed by observed parameter during composting are temperate, pH value, C/N ratio, Kation Exchange Capacity and total phosphor (P) and Potassium (K) contents. This research used Completely Randomized Design with three times repeatation. The treatment of this research is isolate addition which obtained from purification and the controls are EM4 activator addition as positive control and without starter addition (TS) as negative control. Thirteen isolates obtained from the isolation process, eight isolate from waste. and five isolate from rumen substance. Selection ability to decompose cellulose there are two isolates from organic wasting (ISK and ISB) and one from rumen substance (IRK). Test by Composting showed that IRK and ISK isolate caused higher temperate and it also happened in pH value. Isolate ISK and IRK has pH value that almost same as pH value of composting by adding EM4 which become as positive control. Composting result showed that compost reduction velocity at first and second week was significantly different (P<0.05), while at third week it was very significantly different (P<0.01). The obtained of compost production showed that adding EM4 was very significantly different (P<0,01) with compost production that obtained from ISK, IRK and ISB. Compost C/N ratio showed that value of compost kation exchange capacity was significantly different (P<0.05).