Analisis Single Nucleotide Polymorphisms (SNPs) Aksesi Sengon (Falcataria falcata) Toleran dan Intoleran Terhadap Hama Boktor
Date
2024-02-01Author
Pramisari, Yunita
Haneda, Noor Farikhah
Siregar, Ulfah Juniarti
Metadata
Show full item recordAbstract
Sengon memiliki nilai lebih dari segi ekonomi maupun ekologi. Namun,
sengon mudah terserang hama seperti larva boktor. Tujuan penelitian ini adalah
mengevaluasi pertumbuhan sengon di Kesatuan Pemangkuan Hutan (KPH) Kediri,
menganalisis dan menguji hubungan marka SNPs dengan aksesi sengon toleran dan
intoleran terhadap hama boktor. Sampel yang diuji berjumlah 40 aksesi, aksesi
toleran (R28, R29, 230, R33, R47) dan intoleran (S13, S21, S22, S24, S25) dengan
ulangan 4 aksesi tiap aksesi. Setiap sampel diuji menggunakan 4 primer SNPs
dengan metode High-resolution Melting (HRM). Data dianalisis menggunakan uji
Mann-whitney, uji khi-kuadrat, Manhattan plot, dan Principal Component Analysis
(PCA). Nilai keparahan serangan pada aksesi toleran dan intoleran tidak berbeda
nyata. Hasil analisis SNPs menunjukkan terdapat hubungan antara primer yang
digunakan dengan sifat toleran sengon. Hasil visualisasi menunjukkan 4 primer
tersebut mampu memisahkan aksesi sengon toleran dan intoleran, sehingga marka
SNPs ini dapat digunakan untuk seleksi pohon toleran terhadap hama boktor. Sengon has more value from an economic and ecological perspective. However,
sengon is easily attacked by pests such as boktor larvae. The aim of this research was to
evaluate the growth of sengon in Forest Management Unit (KPH) Kediri, analyze and test
the relationship between SNPs markers and tolerant and intolerant sengon accessions to
boktor pests. The samples tested consisted of 40 accessions, tolerant (R28, R29, 230, R33,
R47) and intolerant (S13, S21, S22, S24, S25) with 4 replications for each accession. Each
sample was tested using 4 SNPs primers using the High-resolution Melting (HRM) method.
Data were analyzed using the Mann-Whitney test, chi-square test, Manhattan plot, and
Principal Component Analysis (PCA). The attack severity values for tolerant and intolerant
accessions were not significantly different. The results of the SNPs analysis showed there
was a relationship between the primers used and the tolerant nature of sengon. The
visualization results show that the 4 primers are able to separate tolerant and intolerant
sengon accessions, so that these SNP markers can be used to select trees tolerant to boktor
pests.
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