Pathomorphology of Doxorubicin-Induced Testicular Toxicity in Rat

Abstract

Mangrove merupakan tumbuhan berkayu yang menempati habitat pada daerah yang tergenang air pasang secara periodik. Perubahan iklim yang sedang terjadi dikhawatirkan berdampak pada ekosistem mangrove yang ada di sekitar Kepulauan Karimun Jawa. Penelitian ini bertujuan mengidentifikasi serta memetakan variabel dan kerentanan SES mangrove di Pulau Kemujan dan Pulau Karimun, Kepulauan Karimun Jawa, Provinsi Jawa Tengah. Penelitian dilakukan pada bulan November 2022 di Pulau Kemujan dan Pulau Karimun, Kepulauan Karimun Jawa, Kabupaten Jepara, Provinsi Jawa Tengah. Pengambilan data dilakukan selama 20 hari. Data primer diperoleh melalui teknik metode participatory mapping dan participatory data collection. Keterkaitan variabel SES mangrove di Kepulauan Karimun Jawa, Provinsi Jawa Tengah memiliki hubungan yang kuat antara Resource Governance dengan Resource System. Keterkaitan variabel SES dan variabel kerentanan SES mengindikasikan bahwa masyarakat dan ekosistem mangrove di Kepulauan Karimun Jawa pada kategori rentan terhadap ancaman perubahan iklim.

Doxorubicin, a well-known chemotherapy drug with cytotoxic effects on various cancer cells has raised concerns about its potential harm to normal tissues, especially the male reproductive system. This investigation examines the pathomorphological changes induced by doxorubicin in rodent testes. Male rats were divided into two groups: one received weekly intraperitoneal injections of doxorubicin at 4 mg/kg body weight for four weeks (total dose: 16 mg/kg), while the other received an equivalent volume of sodium chloride (NaCl) solution as a control. After the treatment period, the rats were euthanized, and their testes were carefully dissected for histopathological examination. The analysis revealed significant changes in the doxorubicin-treated group compared to the control group. These changes included seminiferous tubule degeneration, disruption of tissue architecture, and a decrease in spermatogenic cell populations, including spermatogonia, spermatocytes, and spermatids. These findings suggest that doxorubicin administration in rodents causes testicular toxicity through oxidative stress mechanisms. In summary, this study shows how doxorubicin affects rat testes, underscoring its potential harm to male reproductive health. More research is needed to find ways to prevent doxorubicin-induced testicular toxicity.