Keragaman Karakter Xilanase Dari Tiga Isolat Streptomyces Asal Indonesia

Abstract

Xilan is the major component of hemicellulose found in plant cell wall. The aboundance of xylan in nature suggested the importance of xylan bioconvertion by xylanases. Three isolates of Streptomyces from Indonesia respective to 45I-3, 234P-16, and SKK1-8 could produce extracelluler xylanases when grown in birchwood xylan medium. Stability test in optimum temperature showed that xylanases from 234P-16, SKK1-8, and 45I-3 have half life time 23 hour 9 minute, 1 hour 45 minute, and 2 hour 9 minute, respectively. Xylanases from three isolates were stable at temperature 3 oC. Xylanases from three isolates showed the highest activity at oatspelt xylan and the lowest at CMC. HPLC analysis showed that hydrolytic product of birchwood xylan especially are xylosa and arabinosa. Xylanases from three isolates could be precipitated by acetone. Zymogram analysis showed that three isolates could degrade birchwood xylan, and their molecular masses were estimated to be approximately 16.37 kDa, 14.39 kDa, and 12.65 kDa for 45I-3; 16.80 kDa and 14.76 kDa for 234P-16; and 16.80 kDa, 15.21 kDa, and 13.86 kDa for SKK1-8.