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      Peran Bacillus sp. dan Bakteri Penambat Nitrogen dalam Mengendalikan Sclerotium rolfsii dan Menginduksi Pertumbuhan Tanaman Kedelai

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      Date
      2023
      Author
      Arrahmah, Nisa Aulia
      Giyanto
      Suryaningsih, Andika Septiana
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      Abstract
      Tanaman kedelai merupakan tanaman pangan dari kelompok kacang-kacangan yang mampu berasosiasi dengan bakteri penambat nitrogen melalui pembentukan bintil akar. Infeksi patogen menjadi salah satu faktor penting yang memengaruhi kualitas dan kuantitas hasil produksi kedelai, salah satunya yaitu penyakit busuk pangkal batang yang disebabkan oleh Sclerotium rolfsii. Penelitian ini bertujuan mengetahui peran Bacillus sp. dalam mengendalikan S. rolfsii dan peran bakteri penambat nitrogen dari bintil akar dalam menginduksi pertumbuhan tanaman kedelai serta hubungan kompatibilitas kedua isolat bakteri. Penelitian dilakukan dengan uji antagonis Bacillus terhadap S. rolfsii, uji kompatibilitas Bacillus sp. dan isolat bintil akar, uji kemampuan isolat bintil akar dalam menginduksi pertumbuhan tanaman, serta identifikasi secara molekuler. Terdapat dua isolat Bacillus yang yaitu B. subtilis dan B. pseudomycoides yang menghambat pertumbuhan S. rolfsii dan kompatibel terhadap bakteri penambat nitrogen yaitu Enterobacter sp. yang mampu menginduksi pertumbuhan tanaman lebih baik dari perlakuan kontrol. Kata kunci: Bintil akar, Enterobacter, Fabaceae, Penyakit tanaman kedelai
       
      The soybean plant is the main legume crop that has been associated with nitrogen fixation bacteria through the formation of root nodules. Plant disease is an important factor affecting the quality and quantity of soybean production. One of the diseases is stem rot caused by Sclerotium rolfsii. This study aims to determine the role of Bacillus sp. in controlling S. rolfsii and the effects of nitrogen-fixation bacteria from root nodules in inducing the growth of soybean plants and the compatibility of the two bacterial isolates. The research methods included a Bacillus antagonist test against S. rolfsii, a compatibility test between Bacillus sp. and root nodule isolates, a test of the ability of root nodule isolates to induce growth of soybean plants, and molecular identification of Bacillus sp. and nitrogen-fixation bacteria isolates. There were two Bacillus isolates that inhibited the growth of S. rolfsii namely B. subtilis and B. pseudomycoides. The two isolates of Bacillus sp. are compatible with isolates of nitrogen fixation bacteria namely Enterobacter sp. and E. cancerogenus. Keywords: Enterobacter, Fabaceae, Root nodules, Soybean plant diseases
       
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      http://repository.ipb.ac.id/handle/123456789/125717
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      • UT - Plant Protection [2514]

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      Copyright © 2020 Library of IPB University
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      Indonesia DSpace Group 
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