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      Hidrolisis Polisakarida Rumput Laut Caulerpa racemosa dengan Fermentasi Lactobacillus plantarum SK (5)

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      Date
      2023
      Author
      Sudibyo, Muhammad Farid
      Santoso, Joko
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      Abstract
      Hidrolisis polisakarida diketahui dapat meningkatkan aktivitas biologis dengan merubah struktur gugus fungsi polisakarida dan menurunkan bobot molekul. Hidrolisis enzim merupakan metode hidrolisis yang ramah lingkungan karena tidak membentuk produk sampingan yang beracun. Lactobacillus plantarum dapat menghasilkan enzim amilase dan selulase yang dapat digunakan untuk menghidrolisis polisakarida C. racemosa. Penelitian ini bertujuan menentukan waktu fermentasi dan konsentrasi starter terbaik dalam proses hidrolisis polisakarida C. racemosa, serta menentukan konsentrasi glukosa C. racemosa sebelum dan setelah fermentasi. Penelitian dibagi menjadi dua tahap, yaitu tahap I dan tahap II. Lama fermentasi pada tahap I adalah 6 hari, sedangkan pada tahap II adalah 48 jam. Konsentrasi starter yang digunakan adalah 0%, 5%, dan 10%. Hasil analisis menunjukkan bahwa perbedaan konsentrasi starter dan lama fermentasi menyebabkan perubahan nilai total BAL, TAT, gula pereduksi, dan konsentrasi glukosa, serta menurunkan nilai pH. Perlakuan terbaik ditunjukkan pada penambahan starter 5% dengan lama fermentasi 24 jam.
       
      Hydrolysis of polysaccharides is known to increase biological activity by changing the structure of polysaccharide functional groups and reducing molecular weight. Enzyme hydrolysis is an environmentally friendly hydrolysis method because it does not form toxic by-products. Lactobacillus plantarum can produce amylase and cellulase enzymes which can be used to hydrolyse C. racemosa polysaccharides. This research aims to determine the best fermentation time and starter concentration in the C. racemosa polysaccharide hydrolysis process, as well as determine the C. racemosa glucose concentration before and after fermentation. The research was divided into two stages, namely stage I and stage II. The fermentation time in stage I is 6 days, while in stage II it is 48 hours. The starter concentrations used are 0%, 5%, and 10%. The analysis results showed that differences in starter concentration and fermentation time caused changes in the total values of LAB, TAT, reducing sugar and glucose concentration, as well as reducing the pH value. The best treatment was shown by adding 5% starter with a fermentation time of 24 hours.
       
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      http://repository.ipb.ac.id/handle/123456789/125378
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      • UT - Aquatic Product Technology [2463]

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      Copyright © 2020 Library of IPB University
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      Indonesia DSpace Group 
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