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      Analisis Struktur Sekretori, Struktur Anatomi, dan Uji Histokimia Tanaman Pegagan Embun ( Hydrocotyle sibthorpioides)

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      Date
      2023-08
      Author
      Shalihah, Hasna Putri
      Sulistyaningsih, Yohana C.
      Dorly
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      Abstract
      Pegagan embun (Hydrocotyle sibthorpoides) merupakan salah satu tanaman yang sering dikonsumsi sebagai lalapan. Studi fitokimia melaporkan tanaman ini mengandung senyawa metabolit sekunder berupa alkaloid, terpenoid, dan fenol, namun lokasi akumulasinya pada jaringan tanaman belum diketahui. Struktur sekretori pada tanaman ini juga belum pernah dilaporkan. Penelitian ini bertujuan menganalisis struktur anatomi, struktur sekretori, dan lokasi senyawa metabolit sekunder pada jaringan tanaman pegagan embun. Pada tanaman ini stoma dijumpai pada helai daun, tangkai daun dan stolon. Tipe stoma bervariasi, yakni anisositik, parasitik, diasitik, dan anomositik. Struktur unik berupa kumpulan stomata ditemukan epidermis adaksial daun. Tangkai daun dan stolon memiliki endodermis yang tidak mengalami lignifikasi. Struktur sekretori yang ditemukan pada tanaman ini adalah sel idioblas, saluran sekretori, dan trikoma multiseriat. Sel idioblas pada semua organ dan trikoma multiseriat mengakumulasi senyawa lipofilik, flavonoid, alkaloid, terpenoid, dan fenol. Berkas pembuluh pada tangkai daun mengakumulasi senyawa flavonoid, alkaloid, seskuiterpen, monoterpen, dan fenol juga terdeteksi pada berkas pembuluh tangkai daun. Berkas pembuluh pada stolon mengakumulasi senyawa lipofilik, alkaloid, monoterpen, seskuiterpen, dan fenol. Senyawa flavonoid golongan flavonon dan flavon ditemukan pada jaringan tumbuhan pegagan embun.
       
      Dwarf pennywort (Hydrocotyle sibthorpoides) is a is often consumed as raw greens. Phytochemical studies report that this plant contains secondary metabolites in the form of alkaloids, terpenoids, and phenols, but the location of their accumulation in plant tissues is still unknown. The secretory structure of this plant has also not been reported. This study aims to analyze the anatomical structure, secretory structure, and location of secondary metabolites in the plant tissue of dwarf pennywort. In this plant stomata are found on the leaf blade, petiole and stolons. Stoma types are varied, including anisocytic, parasitic, diacytic, and anomocytic. A unique structure in the form of a group of stomata is found in the adaxial epidermis of leaves. The petioles and stolons have a non-lignified endodermis. The secretory structures found in this plant are idioblast cells, secretory ducts, and multiseriate trichomes. Idioblast cells in all organs and multiseriate trichomes accumulate lipophilic compounds, flavonoids, alkaloids, terpenoids, and phenols. Flavonoids, alkaloids, sesquiterpenes, monoterpenes, and phenols were detected in petiole bundles. Vascular bundles in stolons accumulate lipophilic compounds, alkaloids, monoterpenes, sesquiterpenes, and phenols. Flavonoid compounds in the form of the flavonones and flavones were found in this plant tissues.
       
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      http://repository.ipb.ac.id/handle/123456789/124346
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      • UT - Biology [2401]

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