Efektivitas Mikorherbal Daun Kemangi sebagai Antelmintik terhadap Haemonchus contortus pada Pengujian In Vitro

Abstract

Haemonchus contortus merupakan salah satu parasit ruminansia yang patogen dan telah menimbulkan resistensi terhadap pengobatan antelmintik sintetik. Penelitian ini menguji efektivitas antelmintik daun kemangi skala mikron secara in vitro terhadap telur, larva infektif (L3), dan cacing dewasa H. contortus. Efektivitas tiga macam konsentrasi mikroherbal (10, 5, dan 1%) diuji melalui egg hatch assay, larval motility inhibition test, and adult worm motility inhibition assay. Kelompok kontrol negatif menggunakan PBS 10% sementara kontrol positif menggunakan Albendazole 10 mg/ml. Hasil penelitian menunjukkan bahwa perlakuan mikroherbal konsentrasi tertinggi (10%) memiliki efek yang tidak berbeda nyata dengan Albendazole (p>0,05), baik terhadap telur, L3, maupun cacing dewasa. Penghambatan motilitas seluruh cacing dewasa terjadi pada 1-4 jam pertama paparan mikroherbal 10%. Mikroherbal daun kemangi efektif dijadikan antelmintik terhadap berbagai stadium H. contortus.

Haemonchus contortus is one of the prevalent and pathogenic ruminant parasites that has grown resistance to common anthelmintic treatment. This study evaluated the anthelmintic effectiveness of Ocimum africanum leaf on a micron scale against H. contortus eggs, infective stage larvae (L3s), and adult worm. The efficacy of three microherbal concentrations (10, 5, and 1 %) were tested through egg hatch assay, larval motility inhibition test, and adult worm motility inhibition assay. The negative control group used 10% PBS while the positive control used Albendazole 10 mg/ml. The results of this study indicated that the highest concentration (10%) of microherbal treatment had an effect that was not significantly different from Albendazole (p>0.05), both on eggs, L3, and adult worms. Adult worms completely immobilized within the first 1-4 hours of exposure to 10% microherbal. Basil leaves in the form of microherbals were effective as an anthelmintic against various stages of H. contortus.