Pengujian Kemurnian Sapi Bali dengan Menggunakan Metode Isoelektric Focusing

Abstract

The experiment was conducted in order to detect the purity of Bali cattle by examining the hemoglobin tape patterns ontained from Isoelectric focusing methods. The blood samples were taken from five Bali catle from Bali, three bali cattle from West Nusa Tenggara, two Brangus cattle from Tapos, t h e Limousin and three Simmental catles from Australia reared at Balai Inseminasi Buatan Singosari, Malang. Tetrametric hemoglobin and globin subunit sample preparation were used The results indicate that out of eight Bali cattle reared at Balai Inseminasi Buatan Singosari, four of them are not pure. Sample No. 3 which is Bali cattle from NTB had fP ($upta)p e instead of pm,S amples No. 4 and 7 which are Bali cattle from Bali has PB instead fo pb.U,a nd sample No. 9 which is Bali cattle from NTB has no pBali, but has PA. Based on these results, it can be concluded that the bulls have contaminated the purity of Bali cattle in the basic population, including at Bali island. The used of tetrametric hemoglobin method for testing the purity of Bali cattle using Isoelctdc focusing methods show a better result and more efficient when compared to subunit globin method.