Profil Darah Monyet Ekor Panjang (Macaca Fascicularis) Yang Diberi Pakan Berenergi Tinggi Pada Periode Obesitas Empat Bulan Pertama

Abstract

The aim of this research was to determine blood profiles of Long-tailed Macaca (Macaca fascicularis) which were fed with high fat and soluble carbohydrate as the obese diet. The ingredients of obese diet came from local source such as sugar, wheat flour, tallow, vegetable oil, fish meal, maize flour, soybean meal, rice bran, yolk, mineral (calsium carbonat and calsium phosphate) and fiber source from agaragar. Environmental enrichment was alternately given fruit apple, orange, papaya, and guava (weight 10 g/head/day) in a frozen state. Bananas were given about 70 g/day as additional feed. Ten adult males (av. BW 3-5 kg, 5-6,5 year) and five adult males (BW 3-5 kg, 5-6,5 year) subsequently treated with obese diet and control diet. Observed variable are red blood cells count, hemoglobin, hematocrit, Mean Corpuscular Hemoglobin (MCH), Mean Corpuscular Volume (MCV) Mean Corpuscular Hemoglobin Concentration (MCHC) for red bood cell indices and neutrofil, eosinofil, basofil, limfosit and monosit for white blood cell differentiation. The blood was collected from femoral vein under anaesthetized condition. Blood samples were analyzed by automated machine (hematology analyzer). High energy diet had highly significant influence (P<0,01) red blood cell count, hemoglobin, hematocrit, and MCV and less significant for MCHC and white blood cells differentiation (P>0,05). Period nested within feed treatment did not significantly (P>0,05) except on MCHC (highly significant, P<0,01). Duncan test results showed that diet B was the most significant influence and feed A had the same influence with feed to red blood cells count (P<0,05). The influence of feed B significantly higher than the feed A, and feed A feed was higher than feed for hemoglobin and hematocrit (P<0,05). The influence of feed C was significantly higher than feed B, and diet B was higher than feed A (P<0,05) for MCV and MCH. Fourth period was higher influence, 3rd and 2nd period were higher than initial period and 1st period for MCHC. Keywords: Macaca fascicularis, laboratory animal, high energy diet and blood profiles