Integrasi Photosynthesis Microbial Fuel Cell Menggunakan Citrobacter freundii GBH253 dan Chlorella sp. ICBB9005 pada Limbah Cair Tahu

Abstract

Photosynthesis Microbial Fuel Cell (PMFC) integration covering wastewater treatment, electricity production, and microalgae biomass production has prospects to be applied. Electricity production can be increased by combining wastewater as substrate and sodium acetate as co-substrate to increase exoelectrogen microbial activity in transferring extracellular electrons. This study aims to optimize electricity production by combining tofu wastewater as a substrate with sodium acetate as a co-substrate in the growth medium for Citrobacter freundii GBH253 at the anode. Chlorella sp. ICBB9005 is an electron acceptor, utilizing tofu wastewater as a carbon source with photosynthesis at the cathode. The treatment is given in this study various concentrations of sodium acetate co-substrate (0, 10, 20, 30 mM) in tofu wastewater. The research design used was a completely randomized design with an analysis of variance and Tukey's test to determine the significance of the effect between treatments (p<0,05). The results showed that 30 mM sodium acetate co-substrate was added into tofu wastewater 40% (v/v) as a growth media for C.freundii GBH253 5% (v/v) was able to produce maximum output voltage and power density 785,5 mV and 273,43 mW.m-2 at the anode PMFC. Each treatment differs significantly (P<0.05). Chlorella sp. ICBB9005, as an electron acceptor, produced O2 utilizing tofu wastewater as a carbon source with photosynthesis and reduced COD at the cathode PMFC. Utilizing tofu wastewater while producing biomass of Chlorella sp. ICBB9005 is 0.748 g.mL-1 at the cathode PMFC. Based on this study's results, the PMFC uses C. freundii GBH253 and Chlorella sp. ICBB9005 has the integrated to reducing COD and optimizing bioelectricity and biomass microalgae.