Pengaruh Kadar Enzim Papain terhadap Hasil Samping Industri Ikan Sidat (Anguilla bicolor bicolor)
Date
2022-10-05Author
Nuryantina, Renita
Jacoeb, Agoes Mardiono
Nurilmala, Mala
Metadata
Show full item recordAbstract
Hasil samping ikan sidat dapat dikembangkan menjadi produk bernilai
ekonomi tinggi yaitu dengan pembuatan hidrolisat protein. Hidrolisat protein
merupakan hasil penguraian protein menjadi peptida sederhana melalui proses
hidrolisis oleh enzim, asam, atau basa. Tujuan penelitian ini adalah menentukan
pengaruh penambahan enzim papain (1, 2, 3%) terhadap derajat hidrolisis, bobot
molekul dan aktivitas antioksidan serta struktur mikroskopis hidrolisat protein
hasil samping ikan sidat segar dan rebus. Hidrolisat protein dengan perlakuan
segar dan rebus menggunakan enzim papain 1, 2, dan 3% tidak berpengaruh
nyata terhadap derajat hidrolisis. Hidrolisis dilakukan pada suhu 55℃ selama 3
jam dengan waterbath shaker. Derajat hidrolisis tertinggi yaitu pada hidrolisat
segar 3% dan rebus 1%. Bobot molekul hidrolisat protein hasil samping ikan sidat
yang diperoleh berkisar 7-11 kDa. Aktivitas dan kapasitas antioksidan tertinggi
terdapat pada sampel yang telah diberi perlakuan penambahan enzim yakni
hidrolisat segar dan hidrolisat rebus. The by-product of eel can be developed into a product with high economic
value by making protein hydrolysate. Protein hydrolysate is the result of the
breakdown of proteins into simple peptides through the hydrolysis process by
enzymes, acids, or bases. This study aimed to determine the effect of the addition
of papain enzymes (1, 2, 3%) on the degree of hydrolysis, molecular weight, and
antioxidant activity as well as the microscopic structure of protein hydrolysate byproducts
of fresh and boiled eel. Protein hydrolyzate with fresh and boiled
treatment using papain 1, 2, and 3% enzymes did not significantly affect the
degree of hydrolysis. Hydrolysis was carried out at 55℃ for 3 hours with a water
bath shaker. The highest degree of hydrolysis was at 3% fresh hydrolyzate and 1%
boiled. The molecular weight of the protein hydrolyzate by-product of eel
obtained ranged from 7-11 kDa. The highest antioxidant activity and capacity
were found in samples that had been treated with the addition of enzymes, namely
fresh hydrolyzate, and boiled hydrolyzate.