Isolasi dan Karakterisasi Bakteri Penghasil Biosurfaktan
Date
2022-08Author
Angelica, Angie
Rusmana, Iman
Priyanto, Jepri Agung
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Aktivitas petrogenik dan pirogenik di wilayah pesisir DKI Jakarta, khususnya sungai Cilincing mengakibatkan tercemarnya wilayah perairan oleh senyawa hidrokarbon. Bakteri penghasil biosurfaktan dapat dijadikan sebagai alternatif penangan pencemaran tersebut karena bersifat lebih ramah lingkungan dan toksisitasnya rendah jika dibandingkan dengan surfaktan sintetis. Penelitian ini
bertujuan untuk mengisolasi dan mengarakterisasi bakteri penghasil biosurfaktan dari sampel tanah Sungai Cilincing. Isolasi bakteri dilakukan dengan menggunakan Mineral Salt Media (MSM) dengan menggunakan sumber karbon berupa solar 2%. Karakterisasi dilakukan dengan menguji aktivitas hemolisis, drop-collapse test, dan oil-spreading test. Sebanyak 19 isolat berhasil diisolasi dari sampel tanah Sungai Cilincing yang dibedakan berdasarkan ciri morfologinya. Berdasarkan hasil pewarnaan Gram, 15 isolat tergolong Gram positif dan 4 isolat tergolong Gram negatif. Dari 19 isolat yang mampu tumbuh pada media MSM +2% solar, sebanyak 9 isolat memberi hasil positif pada lebih dari satu uji dan diduga mampu memproduksi biosurfaktan, namun hanya satu isolat yang memberikan hasil negatif pada pengujian aktivitas hemolisis, yaitu isolat STC234. Isolat tersebut memiliki nilai indeks emulsifikasi sebesar 44,665±0,001%. The petrogenic and pyrogenic activities in the coastal areas of DKI Jakarta, especially the Cilincing River, have resulted in the contamination of water areas by hydrocarbon compounds. Biosurfactant-producing bacteria can be used as an alternative solution to handle the pollution because it is more environmental
friendly and has low toxicity compared to synthetic surfactants. This study aims to isolate and characterize biosurfactant-producing bacteria from Cilincing River soil samples. Bacterial isolation was carried out using Mineral Salt Media (MSM) using 2% diesel as a carbon source. Characterization was done by testing the activity of hemolysis, drop-collapse test, and oil-spreading test. Total of 19 isolates were isolated from Cilincing River soil samples which were differentiated based on their morphological characteristics. Based on the results of Gram staining, 15 isolates were classified as Gram positive and 4 isolates classified as Gram negative. From 19 isolates that were able to grow on MSM +2% diesel media, 9 isolates gave positive results in more than one test and assumed to be able to produce biosurfactants, but only one isolate gave negative results on hemolytic activity tests, namely isolate STC234. The isolate had an emulsification index value of 44.665±0.001%.
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