Kualitas Semen Cair Domba Lokal yang Diistirahatkan dari Program Perkawinan
Abstract
Istirahat kawin dalam waktu lama memengaruhi kualitas semen. Tujuan
penelitian ini adalah mengevaluasi kualitas semen cair domba lokal Indonesia yang
diistirahatkan kawin. Semen dikoleksi dari empat ekor domba menggunakan vagina
buatan. Semen segar dievaluasi secara makroskopis dan mikroskopis. Semen
kemudian diencerkan dengan pengencer Tris-fruktosa-kuning telur (Tris) atau Na
sitrat-fruktosa-kuning telur (Na sitrat) hingga konsentrasi 50x106 sel/0,2 mL.
Semen cair dikemas dalam tabung sentrifugasi dan disimpan dalam lemari
pendingin (4−5
◦C). Motilitas progresif dan viabilitas spermatozoa semen cair
dievaluasi setiap 24 jam selama 4 hari. Pengaruh lingkar skrotum (LS) terhadap
semen segar maupun semen cair juga dievaluasi. Hasil penelitian menunjukkan
bahwa semen segar memiliki volume 1,08±0,12 mL, warna putih susu, konsistensi
encer hingga kental, pH 6,42±0,13, gerakan massa 2,94±0,13, konsentrasi
4941±926,84x106
/mL, motilitas progresif 79,38±2,17%, viabilitas 87,74±3,48%,
dan abnormalitas total 7,99±3,14%. Istirahat kawin meningkatkan konsentrasi dan
abnormalitas spermatozoa semen segar. LS memengaruhi motilitas, viabilitas,
konsentrasi, dan abnormalitas spermatozoa semen segar. Namun, istirahat kawin
tidak memengaruhi kualitas semen cair. Tris mempertahankan motilitas dan
viabilitas spermatozoa semen cair lebih baik dibandingkan dengan Na sitrat.
Motilitas dan viabilitas spermatozoa semen cair kelompok LS 29 cm lebih baik
dibandingkan dengan kelompok LS 23 cm. Istirahat kawin disimpulkan
memengaruhi kualitas semen segar, namun tidak memengaruhi kualitas semen cair
domba lokal. A long period of sexual rest influences the semen quality. The objective of
this study is to evaluate the quality of chilled Indonesian local ram semen after it
was rested from the breeding program. Semen was collected from four local rams
using an artificial vagina. Fresh semen was examined macroscopically and
microscopically. Semen was diluted in Tris-fructose-egg yolk (Tris) or sodium
citrate-fructose-egg yolk (Na citrate) to a final concentration of 50x106 cell/0.2 mL.
Chilled semen was packed in a centrifugal tube and stored in the refrigerator
(4−5◦C). Sperm progressive motility and viability of chilled semen were examined
every 24 hours for 4 days. The effect of the scrotal circumference (SC) on fresh and
chilled semen was also investigated. The results showed that the fresh semen had a
volume of 1.08±0.12 mL, milky-white color, thin to thick consistency, pH of
6.42±0.13, mass movement of 2.94±0.13, concentration of 4941±926.84x106
sperm/ mL, progressive motility of 79.38±2.17%, viability of 87.74±3.48%, and
total abnormality of 7.99±3.14%. Sexual rest increased fresh semen concentration
and sperm abnormality. SC affected sperm motility, viability, concentration, and
abnormality of ram fresh semen. Sexual rest did not affect the quality of chilled
semen. Tris extenders preserved viability and motility of ram sperm better than Na
citrate. The sperm motility and viability in chilled semen from SC of 29 cm were
better than semen from SC of 23 cm. This study concluded that sexual rest had
effects on the fresh semen but showed no significant effect on chilled local ram
semen.