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      Kualitas Mikrobiologi Pakan Hewan Peliharaan Berbahan Dasar Daging Mentah Berdasarkan Jumlah Enterobacteriaceae dan Escherichia coli

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      Date
      2022
      Author
      Putri, Oktaviani
      Pisestyani, Herwin
      Soviana, Susi
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      Abstract
      Daging mentah untuk pakan hewan peliharaan (raw meat-based diets/RMBDs) dianggap memiliki nutrisi alami yang lebih baik dibandingkan dengan pakan olahan seperti wet food dan dry food. Penularan mikroorganisme patogen yang dapat mengganggu kesehatan manusia dan hewan dapat terjadi apabila higiene dan sanitasi dalam pengolahan RMBDs, terutama dalam pengemasan, penyimpanan, pengiriman dan penyajian tidak dilakukan dengan baik.Penelitian ini bertujuan mengetahui kualitas mikrobiologi pakan hewan peliharaan berbahan dasar daging mentah berdasarkan jumlah Enterobacteriaceae dan E. coli. Sebanyak 3 merek RMBDs dengan 5 kali ulangan dan 1 merek wet dog food dengan 6 kali ulangan, berbahan dasar daging ayam dan sapi dibeli secara online dari marketplace. Pengujian Enterobacteriaceae menggunakan metode hitungan cawan dan metode most probable number (MPN) untuk menghitung jumlah E. coli. Hasil penelitian ini menunjukkan jumlah Enterobacteriaceae dan E. coli dalam RMBDs lebih tinggi dibandingkan dengan wet dog food. Rata-rata jumlah Enterobacteriaceae dalam RMBDs ayam, RMBDs sapi, dan wet dog food berturut-turut adalah 3,2 × 104 ± 4,8 × 104 CFU/g, 2,8 × 103 ± 2,6 × 103 CFU/g, dan 4,5 × 101 ± 6,4 × 101 CFU/g. Rata-rata jumlah E. coli dalam RMBDs ayam, RMBDs sapi, dan wet dog food berturut-turut yaitu 12,98 ± 16,34 MPN/g, 10,71 ± 14,63 MPN/g, dan 0 MPN/g. Tidak terdapat hubungan yang signifikan antara jenis daging dan jumlah Enterobacteriaceae dalam RMBDs. Jumlah Enterobacteriaceae dan E. coli dalam RMBDs belum memenuhi standar yang telah ditetapkan.
       
      Raw meat-based diets (RMBDs) are nutritionally superior compared to wet food and dry food. Transmission of pathogenic microorganisms that interfere with human and animal health occured if hygiene and sanitation in the processing of RMBDs for pets carried out improperly, especially in packaging, storage, delivery and serving. This research aimed to determine the microbiological quality of RMBDs for pets based on total of Enterobacteriaceae and E. coli. A total of 3 brands of RMBDs with 5 replications and 1 brand of wet dog food with 6 replications, the basic ingredients of chicken and beef were purchased from online marketplace. Plate count method was used for Enterobacteriaceae counting and E. coli counted by using the most probable number (MPN). The result showed that the total of Enterobacteriaceae and E. coli was higher in RMBDs compared to wet dog food. The average of Enterobacteriaceae in chicken RMBDs, beef RMBDs, and wet dog food were 3,2 × 104 ± 4,8 × 104 CFU/g, 2,8 × 103 ± 2,6 × 103 CFU/g, and 4,5 × 101 ± 6,4 × 101 CFU/g respectively. The average of E. coli in chicken RMBDs, beef RMBDs, and wet dog food were 12,98 ± 16,34 MPN/g, 10,71 ± 14,63 MPN/g, and 0 MPN/g respectively. There is no significant correlation between the type of meat and the total of Enterobacteriaceae of RMBDs. The total of Enterobacteriaceae and E. coli of RMBDs has not met the maximum standart.
       
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      http://repository.ipb.ac.id/handle/123456789/112648
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      • UT - Animal Disease and Veterinary Health [1241]

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