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      • UT - Faculty of Fisheries and Marine Science
      • UT - Aquatic Product Technology
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      Karakteristik Protein dan Struktur Jaringan serta Steroid Ikan Sidat (Anguilla bicolor-bicolor) Berdasarkan Lokasi Daging Berbeda

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      Date
      2022-02
      Author
      Sinaga, Yanti
      Jacoeb, Agoes Mardiono
      Nurilmala, Mala
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      Abstract
      Ikan sidat merupakan ikan konsumsi yang memiliki rasa yang unik serta kaya akan vitamin A, B1, B2, B6, C, D, protein albumin, DHA dan EPA yang lebih dikenal dengan omega-3, serta kandungan mineralnya. Penelitian bertujuan menentukan kandungan protein dan steroid serta struktur jaringan di berbagai bagian tubuh yang berbeda pada ikan sidat (Anguilla bicolor bicolor). Ikan sidat dianalisis pada tiga bagian tubuh yang berbeda meliputi bagian depan, tengah, dan belakang. Profil protein diidentifikasi dengan metode SDS-PAGE dan Bradford, steroid dengan metode ekstraksi, struktur jaringan dengan metode mikoskopis. Analisis SDS-PAGE pada daging ikan sidat memiliki bobot molekul protein berkisar 9,825–199,196 kDa. Kadar protein terlarut pada bagian depan, tengah, belakang secara berturut-turut 0,307±0,02 mg/mL, 0,298±0,00 mg/mL, dan 0,349±0,05 mg/mL. Protein larut air dan larut garam pada bagian depan, tengah, belakang secara berturut-turut 0,469±0,01 mg/mL, 0,336±0,03 mg/mL, 0,437±0,00 mg/mL dan 1,571±0,16 mg/mL, 1,312±0,11 mg/mL, 1,242±0,11 mg/mL. Ikan sidat mengandung steroid pada pelarut etil asetat. Struktur jaringan daging dan kulit ikan sidat terdapat muscle fiber dan lapisan stratum compactum.
       
      Eel is a consumable fish that has a unique taste and is rich in vitamins A, B1, B2, B6, C, D, protein albumin, DHA and EPA which is better known for omega-3, as well as its mineral content. This research aimed to determine the protein and steroid content and tissue structure in various parts of the body in eel fish (Anguilla bicolor bicolor). Eels are analyzed on three different body parts covering the front, central, and back. The protein profile was identified by the SDS-PAGE and Bradford methods, steroids by extraction method, tissue structure by the microscopic method. SDS-PAGE analyzed on eel fish meat has a protein molecular weight ranging from 9.825–199.196 kDa. Dissolved protein levels at the front, central, back are 0.307±0.02 mg/mL, 0.298±0.00 mg/mL, and 0.349±0.05 mg/mL. Water soluble and salt soluble proteins at the front, central, back are 0.469±0.01 mg/mL, 0.336±0.03 mg/mL, 0.437±0.00 mg/mL and 1.571±0.16 mg/mL, 1.312±0.11 mg/mL, 1.242±0.11 mg/mL. Eel fish contained steroids in ethyl acetate solvents. The structure of meat tissue and eel fish skin contained muscle fiber and stratum compactum layer.
       
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      http://repository.ipb.ac.id/handle/123456789/111211
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      • UT - Aquatic Product Technology [2463]

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      Copyright © 2020 Library of IPB University
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      Indonesia DSpace Group 
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