Karakterisasi Kimiawi Spirulina Fermentasi dan Penapisan Senyawa Metabolit yang Dihasilkannya
Abstract
Spirulina platensis dapat menstimulasi pertumbuhan mikroba non
patogenik. Penelitian sebelumnya menunjukkan Spirulina fermentasi memiliki
karakteristik kimiawi dan aktivitas antioksidan. Pengeringan beku (liofilisasi)
dilakukan dan digunakan dalam penelitian ini. Penelitian bertujuan menentukan
karakteristik kimiawi dan penapisan senyawa metabolit dari Spirulina fermentasi
setelah liofilisasi. Spirulina fermentasi liofilisasi memiliki nilai pH 4,15 ± 0,00,
total asam tertitrasi (TAT) 11,96 ± 0,00 %, dan total bakteri asam laktat (BAL)
<2,5 × 101 ± 0,00. Spirulina fermentasi mengandung komponen aktif flavonoid,
steroid, tanin, saponin dan fenol hidrokuinon, serta negatif alkaloid. Penapisan
senyawa metabolit menggunakan LC-MS/MS menghasilkan 88 senyawa, terdiri
dari golongan terpen atau terpenoid, fenolik, dan alkaloid, serta kelompok asam
amino, asam lemak, asam organik, natural pigmen, gula dan kelompok lainnya.
Senyawa dominan teridentifikasi diantaranya propenyl pyrazine, ribonic acid,
pyroglutamic acid, phenyllactic acid, 1,2-cyclohexanedicarboxylic acid, didecyl
ester, cis-caftaric acid, malic acid, 1,2-cyclohexanedicarboxylic acid,
cyclohexylmethylnonyl ester, dan gallic acid. Spirulina platensis can stimulate the growth of non-pathogenic microbes.
Previous studies have shown that lyophilized fermented Spirulina and Spirulina
biomass have antioxidant activity. The study aims to determine the chemical
characteristics and screening of metabolites produced by lyophilized fermented
Spirulina and Spirulina biomass. Lyophilized fermented Spirulina had a pH value
of 4,15 ± 0,00, total titrated acid (TAT) 11,96 ± 0,00%, and total lactic acid
bacteria (LAB) < 2,5 × 101 ± 0,00. Fermented Spirulina contains active
components of flavonoids, steroids, tannins, saponins and phenol hydroquinone,
as well as negative alkaloids. Screening of metabolites using LC-MS/MS resulted
in 88 compounds, consisting of terpenes or terpenoids, phenolics, and alkaloids,
as well as groups of amino acids, fatty acids, organic acids, natural pigments,
sugars and other groups. The dominant compounds identified were propenyl
pyrazine, ribonic acid, pyroglutamic acid, phenyllactic acid, 1,2-
cyclohexanedicarboxylic acid, didecyl ester, cis-caftaric acid, malic acid, 1,2-
cyclohexanedicarboxylic acid, cyclohexylmethylnonyl ester, and gallic acid.