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      Seleksi dan Karakterisasi Bakteri Penghambat Pertumbuhan Fusarium sp. Asal Tanaman Tomat (Solanum lycopersicum L.).

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      Date
      2022
      Author
      Ayni, Qurrotu
      Mubarik, Nisa Rachmania
      Manaf, Lisdar A
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      Abstract
      One of the problems in tomato (Solanum lycopersicum L.) farming is the emergence of wilt disease, which is caused by the fungal pathogen Fusarium sp. Pathogenic fungi have been controlled by spraying synthetic fungicides. The use of fungicides has a negative impact on other organisms and the environment. The negative impact of excessive use of synthetic chemicals can be reduced by using biocontrol agents that produce antifungal compounds. Efforts to control pathogenic fungi naturally using rhizosphere bacteria to control the growth of pathogenic fungi on tomato plants (Solanum lycopersicum L) have not been widely reported. This study aims to select rhizosphere bacteria from 14 bacterial isolates that have been isolated from tomato farming which are able to inhibit the growth of Fusarium sp. in vitro and characterize selected bacteria. The results of the bacterial antagonist test against the fungus Fusarium sp. with the dual culture method showed that 6 out of 14 isolates were able to inhibit the growth of the fungus Fusarium sp. Isolates TTSG 2.2, TTSG 2.7, TTSG 3.2, TTSG 3.5, TTSG 3.6, and TCS 3.1 had inhibitory power of 45%. One of the six isolates of rhizosphere bacteria, namely TTSG 3.6, showed a negative reaction in the hemolysis test. These results indicate that the bacterial isolate of TTSG 3.6 has the potential to be used as a control for Fusarium sp. on tomato plants
       
      Salah satu masalah dalam pertanian tomat (Solanum lycopersicum L.) ialah munculnya penyakit layu, yang disebabkan oleh cendawan patogen Fusarium sp. Cendawan patogen selama ini dikendalikan dengan menyemprotkan fungisida sintetik. Pengunaan fungisida berdampak negatif terhadap organisme lain dan lingkungan. Dampak negatif dari penggunaan bahan kimia sintetis berlebihan, dapat dikurangi pengunaannya dengan agens biokontrol yang menghasilkan senyawa anticendawan. Usaha pengendalian cendawan patogen secara alami menggunakan bakteri rizosfer sebagai pengendali pertumbuhan cendawan patogen pada tanaman tomat (Solanum lycopersicum L) masih belum banyak dilaporkan. Penelitian ini bertujuan untuk menyeleksi bakteri rizosfer dari 14 isolat bakteri yang telah diisolasi dari pertanian tanaman tomat yang mampu menghambat pertumbuhan Fussarium sp. secara invitro dan melakukan karakterisasi bakteri terpilih. Hasil uji antagonis bakteri terhadap cendawan Fusarium oxysporum dengan metode dual culture menunjukkan bahwa sebanyak 6 dari 14 isolat mampu menghambat pertumbuhan cendawan Fusarium sp. Isolat TTSG 2.2, TTSG 2.7, TTSG 3.2, TTSG 3.5, TTSG 3.6, dan TCS 3.1 memilki daya hambat 45%. Satu dari enam isolat bakteri rizosfer yaitu TTSG 3.6 menunjukkan reaksi negatif dalam uji hemolisis. Hasil ini menunjukkan bahwa isolat bakteri TTSG 3.6 berpotensi digunakan sebagai pengendali Fusarium sp. pada tanaman tomat
       
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      http://repository.ipb.ac.id/handle/123456789/110684
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      Indonesia DSpace Group 
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