Studi Literatur Biosensor Fenol dengan Biofilm Pseudomonas fluorescens Termodifikasi pada Permukaan Screen Printed Carbon Electrode.

Abstract

Sampel air limbah mengandung senyawa organik aromatik seperti fenol. Fenol merupakan zat polutan prioritas dan berbahaya. Metode deteksi fenol secara konvensional menggunakan alat deteksi seperti kromatografi dan spektrofotometri tidak efektif. Alternatif deteksi fenol yakni menggunakan biosensor fenol dengan metode voltametri siklik. Studi literatur ini bertujuan mengkaji mekanisme tirosinase yang tepat, mengkaji optimasi pengukuran biosensor senyawa fenol dengan biofilm P. fluorescens, mengkaji modifikasi elektrode kerja dengan penambahan supporting material, dan mengkaji hasil kinerja analitik biosensor fenol. Pembentukan biofilm P.fluorescens di permukaan SPCE akan mempermudah imobilisasi enzim tirosinase. Keberhasilan imobilisasi ditandai dengan munculnya arus reduksi dan oksidasi. Penambahan magnetit-PEG akan meningkatkan deteksi biosensor fenol sehingga menghasilkan linearitas sebesar 0,9925 dan 0,9989.

Wastewater samples contain aromatic organic compounds such as phenol. Phenols are the priority and harmful pollutant substances. Conventional phenol detection methods using detection tools such as chromatography and spectrophotometry are ineffective. An alternative to phenol detection is to use phenol biosensors with the cyclic voltammetry method. This literature study aims to examine the proper mechanism of tyrosinase, review the optimization of biosensor measurements of phenol compounds with biofilm P. fluorescens, review the modification of working electrodes with the addition of supporting materials, and review the analytical performance results of phenol biosensors. The formation of P.fluorescens biofilms on the surface of the SPCE will facilitate the immobilization of the enzyme tyrosinase. The success of immobilization is characterized by the emergence of reduction and oxidation currents. The addition of magnetite-PEG will improve the detection of phenol biosensors resulting in the linearity of 0,9925 and 0,9989.