Gambaran Histopatologi Organ Paru-paru Ayam (Gallus domesticus) 48 Jam Setelah Kematian
Date
2021-08-19Author
Gumilang, Mutiara Asa Citra
Harlina, Eva
Handharyani, Ekowati
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Forensik veteriner mempelajari penyebab kematian dan menentukan jarak waktu kematian hewan (post mortem interval/PMI). Penelitin forensik veteriner ini bertujuan untuk mempelajari histopatologi paru-paru ayam broiler 48 jam setelah kematian (post mortem). Sebanyak 75 ekor ayam broiler berumur tujuh hari dieutanasia dengan metode pemutusan sendi atlanto occipitale, selanjutnya dibagi menjadi 25 kelompok yang tiap kelompok terdiri atas 3 ekor ayam. Setiap dua jam kadaver ayam dinekropsi, organ paru-paru dikoleksi untuk dibuat sediaan histopatologi dan diwarnai Hematoksilin-Eosin (HE). Histopatologi jaringan paru-paru dianalisis menggunakan software ImageJ dan datanya diuji dengan metode Pearsons. Hasil pengamatan mikroskopis paru-paru pada jam ke-0 hingga jam ke-48 menunjukkan diameter parabronkus yang bertambah lebar, semakin banyak ruang kosong, epitel lumen parabronkus mengalami deskuamasi dan autolisis, dan lumen parabronkus dipenuhi oleh luruhan sel dan cairan. Korelasi diameter parabronkus terhadap waktu setelah kematian sangat lemah sehingga tidak dapat dijadikan acuan PMI. Perubahan jarak interparabronkus, kepadatan sel parabronkus dan pertumbuhan koloni bakteri terhadap waktu kematian dapat dijadikan acuan PMI. Deskuamasi sel epitel parabronkus dimulai pada jam ke-16 dan bakteri mulai tumbuh pada jam ke-22 setelah kematian. Veterinary forensics studies the causes of animal death and determines the time interval of death (post mortem interval/PMI). This veterinary forensic research aims to study the histopathology of broiler chicken lungs 48 hours after death (post mortem). A total of 75 broiler chickens aged seven days were euthanized by the atlanto occipital joint severing method, then divided into 25 groups, each group consisting of 3 chickens. Every two hours the chicken cadaver was necropsied, the lungs were collected for histopathological preparation and stained with Hematoxylin-Eosin (HE). Histopathology of lung tissue was analyzed using ImageJ software and the results were tested using the Pearsons method. The results of microscopic observations of the lungs at 0 to 48 hours showed that the diameter of the parabronchi was getting wider, there was more space, the epithelium of the parabronchial lumen experienced desquamation and autolysis, and the parabronchial lumen was filled with cell debris and fluid. The correlation of parabronchial diameter with time after death is so weak that it cannot be used as a PMI reference. Changes in interparabronchial distance, parabronchial cell density, and the presence of bacterial clony concerning to time of death can be used as a reference for PMI. Desquamation of parabronchial epithelial cells began at the 16th hour and bacteria began to grow at the 22nd hour.