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      Kejadian Infeksi Plasmodium pada Tarsius spp. dan Nycticebus coucang di Pusat Studi Satwa Primata IPB Melalui Pemeriksaan Molekuler Sampel Feses

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      Date
      2021-08-06
      Author
      Christie, Giovanni
      Isdoni
      Hadi, Upik Kesumawati
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      Abstract
      Penyakit infeksius memiliki pengaruh yang nyata terhadap penurunan populasi primata. Adanya kejadian infeksi Plasmodium pada primata membuat penelitian mengenai malaria semakin sering dilakukan pada primata endemik seperti Tarsius spp. dan Nycticebus coucang. Di penangkaran Pusat Studi Satwa Primata IPB (PSSP IPB), keberadaan Plasmodium pada Tarsius spp. dan N. coucang belum pernah dilaporkan. Pertimbangan etis yang melarang pengambilan darah dan jaringan dari primata, menjadikan sampel feses sebagai pilihan yang lebih aman untuk digunakan. Oleh karena itu, penelitian ini bertujuan mendeteksi keberadaan DNA Plasmodium dari sampel feses Tarsius spp. dan N. coucang di penangkaran PSSP IPB. Sampel feses diambil dari kandang Tarsius spp. (n=8) dan kandang N. coucang (n=6) di penangkaran PSSP IPB. DNA dari sampel feses diamplifikasi dengan nested polymerase chain reaction. Hasil penelitian menunjukkan adanya dugaan kejadian infeksi Plasmodium pada Tarsius spectrum kandang nomor enam, sedangkan pada N. coucang tidak ditemukan infeksi Plasmodium.
       
      Infectious disease has a significant effect on decreasing the population of primate. The infection of a primate with Plasmodium iniated resurgence of Non-Human Primate malaria research on endemic primate such as Tarsius spp. and Nycticebus coucang. Ethical considerations that prohibit the collection of blood and tissue from primate, making stool sample as a safer option for use. In captivity Primate Research Center IPB (PSSP IPB), there are no recent studies on Plasmodium infection in Tarsius spp. and N. coucang. Thus, the aim of this study was to detect DNA of Plasmodium from the stool of Tarsius spp. and N. coucang in captivity PSSP IPB. Stool samples were collected from Tarsius spp. cages (n=8) and N. coucang cages (n=6) in capticity PSSP IPB. Extracted DNA from stool samples was used for molecular diagnosis of Plasmodium infection by nested polymerase chain reaction. The result of this study showed that the Tarsius spectrum in cage number six was suspected to be infected with Plasmodium whereas in N. coucang there was no Plasmodium infection.
       
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      http://repository.ipb.ac.id/handle/123456789/108192
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      • UT - Anatomy, Phisiology and Pharmacology [1047]

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      Copyright © 2020 Library of IPB University
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      Contact Us | Send Feedback
      Indonesia DSpace Group 
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      Universitas Jember Digital Repository