Diferensial Leukosit Tikus (Rattus norvegicus) yang diberi Mikrokapsul Imunoglobulin-G Anti H5N1 dan Diinfeksi Virus AI H5N1 Isolat Indonesia

Abstract

Avian Influenza caused by Avian Influenza virus H5N1 is a zoonotic disease for which there is no drug or vaccine available yet. The immunotherapy using IgG anti AI H5 can be an alternative therapy. The microcapsule contain IgG anti AI H5N1 have been designed for in ovo test, the result showed that the microcapsule has high potential to neutralized AI H5N1. Furthermore this study was a in vivo examination that design for efficacy test was conducted in Rat. The hematological profile of animal model was evaluated to understand the microcapsule IgG anti AI H5N1 capability in vivo which has never been reported. This research aimed to study the profile of leukocytes differential of rats (Rattus novergicus) that was infected by the H5N1 Avian influenza virus after treated by using anti-H5N1 IgG microcapsules. As much as 54 male rats, with aged 3 months were divided into three groups. The first group was a control group (K (-)) which only given PBS as placebo., second group infected by AI H5N1 virus (K (+)), and thelast group were administered by IgG anti H5N1 microcapsules and infected with the AI H5N1 virus (KP). The leucocyte were count under microscope to determine the leukocyte differential. Blood smear samples were taken at 0, 36, and 72 hours. The data were analyzed by ANOVA method and Duncan's analysis test in 95% confidence level. The results of the observations showed a tendency to decrease the percentage of lymphocytes ofrat that were given microcapsules and infected (KP) at 72 hours compared to other groups. Rat that were infected with the H5N1 virus only (K+) there was a tendency to decrease the percentage of monocytes and increase the percentage of neutrophils compared to the other two groups. However, the decrease and increase in the percentage of lymphocytes, monocytes and neutrophils in each group were not statistically significant. The number of basophils and eosinophils was not significantly different between the three groups because the percentages were normal. The conclusion of this study was the other leukocyte percentage change which found in H5N1 viral infection were well corrected by giving anti H5N1 microcapsule prior infection.

Keywords: mice, microcapsules, H5N1 virus, leukocyte differential.

Avian Influenza caused by Avian Influenza virus H5N1 is a zoonotic disease for which there is no drug or vaccine available yet. The immunotherapy using IgG anti AI H5 can be an alternative therapy. The microcapsule contain IgG anti AI H5N1 have been designed for in ovo test, the result showed that the microcapsule has high potential to neutralized AI H5N1. Furthermore this study was a in vivo examination that design for efficacy test was conducted in Rat. The hematological profile of animal model was evaluated to understand the microcapsule IgG anti AI H5N1 capability in vivo which has never been reported. This research aimed to study the profile of leukocytes differential of rats (Rattus novergicus) that was infected by the H5N1 Avian influenza virus after treated by using anti-H5N1 IgG microcapsules. As much as 54 male rats, with aged 3 months were divided into three groups. The first group was a control group (K (-)) which only given PBS as placebo., second group infected by AI H5N1 virus (K (+)), and thelast group were administered by IgG anti H5N1 microcapsules and infected with the AI H5N1 virus (KP). The leucocyte were count under microscope to determine the leukocyte differential. Blood smear samples were taken at 0, 36, and 72 hours. The data were analyzed by ANOVA method and Duncan's analysis test in 95% confidence level. The results of the observations showed a tendency to decrease the percentage of lymphocytes ofrat that were given microcapsules and infected (KP) at 72 hours compared to other groups. Rat that were infected with the H5N1 virus only (K+) there was a tendency to decrease the percentage of monocytes and increase the percentage of neutrophils compared to the other two groups. However, the decrease and increase in the percentage of lymphocytes, monocytes and neutrophils in each group were not statistically significant. The number of basophils and eosinophils was not significantly different between the three groups because the percentages were normal. The conclusion of this study was the other leukocyte percentage change which found in H5N1 viral infection were well corrected by giving anti H5N1 microcapsule prior infection.