Resistansi Escherichia coli dan Staphylococcus aureus dalam Susu dari Peternakan Sapi Perah Pondok Ranggon terhadap Antibiotik

Abstract

Penggunaan antibiotik berlebih dapat menyebabkan resistansi antibiotik pada ternak yang dapat disebarkan ke manusia dan lingkungan. Penelitian ini bertujuan mengisolasi bakteri Escherichia coli dan Staphylococcus aureus dari susu segar dan mengetahui sifat resistansinya terhadap antibiotik. Pengujian diawali dengan isolasi dan identifikasi bakteri berdasarkan SNI 2897:2008 tentang Metode Pengujian Cemaran Mikroba dalam Daging, Telur dan Susu, serta Hasil Olahannya dan dilanjutkan dengan pengujian resistansi antibiotik menggunakan metode Kirby-Baurer. Antibiotik yang digunakan yaitu golongan β-laktam, aminoglikosida, makrolida, fluorokuinolon, antagonis asam folat, kloramfenikol, tetrasiklin dan cephems. Dari 36 sampel susu segar didapatkan 16 sampel (25 isolat) positif tercemar E. coli dan 13 sampel (13 isolat) positif S. aureus. Sebanyak 24/25 isolat E. coli resistan terhadap 15 jenis antibiotik dan 13/13 isolat S. aureus resistan terhadap 14 jenis antibiotik. Sebanyak 20/25 isolat E. coli telah mengalami multidrug resistance antibiotic dengan pola yang paling sering ditemui yakni penisilin-aminoglikosida-tetrasiklin-kloramfenikol. Sebanyak 8/13 isolat S. aureus mengalami multidrug resistance dengan pola yang paling sering ditemui yakni penisilin-aminoglikosida-fluorokuinolon. Bakteri yang telah mengalami resistansi antibiotik dapat menjadi ancaman bagi kesehatan ternak, kesehatan lingkungan, keamanan pangan dan kesehatan masyarakat.

Overuse of antibiotics can cause antimicrobial resistance in livestock which can spread to humans and the environment. This study aimed to isolate Escherichia coli and Staphylococcus aureus in raw milk and determined their antibacterial susceptibility. Bacterial isolation and identification based on SNI 2897:2008 about Metode Pengujian Cemaran Mikroba dalam Daging, Telur dan Susu, serta Hasil Olahannya were followed up with an antibacterial susceptibility test using the Kirby-Baurer method. The antibiotics used in this study are β-lactam, aminoglycoside, macrolide, fluoroquinolones, folic acid antagonist, chloramphenicol, tetracycline, and cephems Out of 36 raw milk samples, 16 samples (25 isolates) were contaminated with E. coli and 13 samples (13 isolates) were contaminated with S. aureus. As many as 24/25 of E. coli isolates were resistant to 15 types of antibiotics and 13/13 of S. aureus isolates were resistant to 14 types of antibiotics. As many as 20/25 of E. coli isolates were multidrug-resistant with penicillin-aminoglycoside-tetracycline-chloramphenicol as the most common pattern and 8/13 of S. aureus isolates were multidrug-resistant with penicillin-aminoglycoside-fluoroquinolone as the most common pattern. Antimicrobial resistance can be a threat to animal health, environmental health, food safety, and public health.