Integrasi Sentrifugasi Suhu Rendah dengan Optimasi Ekstraksi Enzimatis Minyak Mata Tuna Menggunakan Response Surface Methodology
Date
2021Author
Kurniawan, Ikram Abi Hamzah
Trilaksani, Wini
Riyanto, Bambang
Metadata
Show full item recordAbstract
Minyak mata tuna yang kaya akan asam lemak omega-3 Eicosapentaenoic
acid dan Docosahexaenoic acid dapat diperoleh dengan ekstraksi suhu rendah
tetapi rendemen yang diperoleh belum maksimal. Nilai rendemen minyak mata tuna
dapat ditingkatkan dengan hidrolisis enzimatis. Response surface methodology
(RSM) dapat membantu menganalisis pengaruh faktor-faktor terhadap respon
untuk mengoptimalkan kondisi hidrolisis enzimatis. Tujuan penelitian ini adalah
maksimalisasi rendemen minyak, menentukan kondisi optimum ekstraksi enzimatis
minyak mata tuna dengan faktor konsentrasi papain dan waktu hidrolisis yang
berbeda menggunakan permodelan central composite design-face centered
(CCDFC) pada RSM, dan determinasi karakteristik minyak. Kondisi hasil validasi
optimum hidrolisis enzimatis minyak mata tuna menghasilkan nilai rendemen
11.24±0.09% pada konsentrasi 1.02% enzim papain selama 3 jam. Kandungan EPA
dan DHA minyak mata tuna pada kondisi optimum tergolong sangat tinggi yaitu
6.02% EPA dan 30.30% DHA dari 99.90% total asam lemak yang teridentifikasi. Tuna eye oil which is rich in omega-3 fatty acids Eicosapentaenoic acid and
Docosahexaenoic acid can be obtained by low temperature extraction however the
yield was not optimum yet. The yield value of tuna eye oil can be increased with
enzymatic hydrolysis. Response Surface Methodology (RSM) can help analyze the
effect of factors on the response to optimize the conditions of enzymatic hydrolysis.
The purpose of this study was to maximization of oil yield, determine the optimum
conditions for enzymatic extraction of tuna eye oil with different papain enzyme
concentration and hydrolysis time using central composite design-face centered
(CCDFC) modeling on RSM and determination of oil characteristics. The
validation optimum of the enzymatic hydrolysis resulted in a yield value of
11.24±0.09% at the concentration of 1.02% papain enzyme for 3 hours. The EPA
and DHA content of tuna eye oil at the optimum condition was classified as very
high, 6.02% EPA and 30.30% DHA from 99.90% of the total identified fatty acids.