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Title: | Isolasi, Seleksi, dan Aplikasi Bakteri Methylotroph dengan Mikoriza dan Pupuk Anorganik terhadap Pertumbuhan Jagung Manis (Zea mays saccharata Sturt) |
Other Titles: | Isolation, Selection, and Application of Methylotroph Bacteria with Mycorrhizae and Inorganic Fertilizers on the Growth of Sweet Corn (Zea mays saccharata Sturt) |
Authors: | Santosa, Dwi Andreas Hazra, Fahrizal Dwimasta, Khannegara Aulia |
Issue Date: | 2023 |
Publisher: | IPB University |
Abstract: | Beberapa dari mikrob yang menarik untuk dikaji sebagai pupuk hayati
adalah bakteri methylotroph dan mikoriza. Penelitian ini bertujuan mengisolasi,
menyeleksi, dan mengidentifikasi bakteri methylotroph, membuat propagul
mikoriza dengan perbanyakan mikoriza, dan mengkaji pengaruh pemberian bakteri
methylotroph, mikoriza, serta dosis pupuk NPK terhadap pertumbuhan tanaman
jagung manis. Penelitian menggunakan Rancangan Acak Lengkap (RAL) Faktorial
yang terdiri dari tiga faktor. Faktor pertama yaitu dosis pupuk NPK dengan 3 taraf
(D0: 0%, D1: 50%, D2: 100% dosis rekomendasi), faktor kedua adalah pemberian
bakteri methylotroph dengan 2 taraf (B0: tanpa bakteri, B1: diberi bakteri), dan
faktor ketiga yaitu pemberian propagul mikoriza dengan 2 taraf (M0: tanpa
propagul, M1: diberi propagul). Data dianalisis secara statistik menggunakan
ANOVA pada taraf 5% dan diuji lanjut dengan DMRT. Hasil penelitian
menunjukkan bahwa bakteri methylotroph berhasil diisolasi, diseleksi, dan identik
dengan Methylophilus methylotrophus ICBB 9931 dengan kemampuan membantu
perkecambahan sebesar 64%. Perbanyakan mikoriza menghasilkan propagul
dengan derajat kolonisasi sebesar 91,1% dan kepadatan spora sebesar 46,5/20 g
media. Hasil percobaan lapang menunjukkan bahwa perlakuan 100% dosis
rekomendasi NPK dengan diberi mikoriza secara tunggal (D2B0M1) memiliki nilai
terbaik pada parameter tinggi tanaman, bobot basah akar, serta bobot kering
tanaman dan akar. Pemberian bakteri Methylophilus methylotrophus ICBB 9931
tidak selalu memberikan hasil yang positif pada tanaman diduga karena kurangnya
bahan organik. Some interesting microbes to be study as biofertilizers are methylotroph bacteria and mycorrhizae. This study aims to isolate, select, and identify methylotroph bacteria, produce mychorrhizae propagules with mycorrhizae propagation, and examine the effect of giving methylotroph bacteria, mycorrhizae, and doses of NPK fertilizer on the growth of sweet corn plants. The study used a completely randomized design (CRD) factorial consist of three factors. The first factor was the dose of NPK fertilizer consist of 3 levels (D0: 0%, D1: 50%, D2: 100% recommended dose), the second factor was the addition of methylotroph bacteria consist of 2 levels (B0: without bacteria, B1: given bacteria), and the third factor is the addition of mycorrhizal propagules consist of 2 levels (M0: without propagules, M1: given propagules). Data were analyzed statistically using ANOVA at the 5% level and it was further tested with DMRT. The results of the study succeeded in isolating and selecting the methylotroph bacteria which were identical to Methylophilus methylotrophus ICBB 9931 with the ability to promote seed germination rates by 64%. Mycorrhizal propagation produced propagules with a colonization degree of 91.1% and a spore density of 46.5/20 g media. The results of the field experiments showed that the 100% recommended dose of NPK treatment with mycorrhizae in particular (D2B0M1) had the best values for the parameters of plant height, fresh weight of roots, and dry weight of plants and roots. The addition of Methylophilus methylotrophus ICBB 9931 does not always give positive results to plants presumably due to the lack of organic matter. |
URI: | http://repository.ipb.ac.id/handle/123456789/116502 |
Appears in Collections: | UT - Soil Science and Land Resources |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Cover.pdf Restricted Access | Cover | 364.62 kB | Adobe PDF | View/Open |
A14180086_Khannegara Aulia Dwimasta.pdf Restricted Access | Full Text | 998.39 kB | Adobe PDF | View/Open |
Lampiran.pdf Restricted Access | Lampiran | 291.45 kB | Adobe PDF | View/Open |
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