View Item 
      •   IPB Repository
      • Dissertations and Theses
      • Undergraduate Theses
      • UT - Faculty of Forestry
      • UT - Silviculture
      • View Item
      •   IPB Repository
      • Dissertations and Theses
      • Undergraduate Theses
      • UT - Faculty of Forestry
      • UT - Silviculture
      • View Item
      JavaScript is disabled for your browser. Some features of this site may not work without it.

      Variasi Genetik Rotan Berdasarkan Penanda DNA Barcode matK, rbcL dan ITS Pada Pangkalan Data GenBank.

      Thumbnail
      View/Open
      Full text (16.92Mb)
      Date
      2015
      Author
      Putri, Miranti Arum
      Siregar, Iskandar Z
      Metadata
      Show full item record
      Abstract
      Species identification of rattan is usually done by assessing morphological characteristics. Along with the development of science and technology, biomolecular science began to be applied as a complementary tool identification, one of which is DNA barcoding technique. In the forestry both are still rarely applied as complementary to each other because of limited molecular data. This study was aimed to analyze the genetic structure of various rattan by three DNA Barcode markers that are available in the genetic database (NCBI), namely matK, rbcL and ITS. Data processing was performed using software MEGA and DnaSP. The results showed that markers of ITS have a longer sequence that is above 500 bp and higher GC content (%) 63.1% compared to the others. Constructed phylogeny trees do not show the most accurate marker in discriminating species because different species are still grouped into one clade. Nucleotide variation analysis was conducted to determine more accurate marker based on genetic variation approach to differentiate Calamus and non-Calamus. However, uncertainty in discriminating species was still observed.
      URI
      http://repository.ipb.ac.id/handle/123456789/78350
      Collections
      • UT - Silviculture [939]

      Copyright © 2020 Library of IPB University
      All rights reserved
      Contact Us | Send Feedback
      Indonesia DSpace Group 
      IPB University Scientific Repository
      UIN Syarif Hidayatullah Institutional Repository
      Universitas Jember Digital Repository
        

       

      Browse

      All of IPB RepositoryCollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

      My Account

      Login

      Application

      google store

      Copyright © 2020 Library of IPB University
      All rights reserved
      Contact Us | Send Feedback
      Indonesia DSpace Group 
      IPB University Scientific Repository
      UIN Syarif Hidayatullah Institutional Repository
      Universitas Jember Digital Repository