Microbiological Characteristic and Antimicrobial Activity of Koumiss Against Salmonella typhimurium and Mycobacterium tuberculosis

Abstract

Koumiss is a traditional fermented milk product originated from the Central Asian steppes and mostly produced from mare milk by spontaneous fermentation of lactose to lactic acid and alcohol. Koumiss’s starter cultures consist of lactic acid bacterias (Lc. lactis and Lb. acidophilus) and yeast (Sc. cereviceae). Koumiss is believed to pose health promoting properties, which is mainly related to the ability of the starter to produce vitamins of the B-group and antimicrobials. Koumiss could also served as functional food that was showed by its capability to produce the antimicrobial substrate that inhibit pathogenic bacterias. The objectives of this research were to identify microbiological characteristics of koumiss and to study its antimicrobial activity towards pathogenic bacterias such as S. Typhimurium ATCC 14028 and M. tuberculosis H37RV. The experimental design used on this research were non parametric of cohran test for M. tuberculosis H37RV and descriptive analysis for S. Typhimurium ATCC 14028. Variables observed were diameter of inhibition zone of the antagonistic assay used well diffusion method agar for S. Typhimurium ATCC 14028. In addition, the Lowenstein Jensen (LJ) modification agar was used for study the inhibition of M. tuberculosis H37RV. The result showed, koumiss could decrease the total of coliform. The average of koumiss’s inhibition zone in different storage time toward S. Typhimurium ATCC 14028 was ± 7.801 mm. It was bigger than filtrate which was ± 6.002 mm. The average of diameter showed the antimicrobial activity of koumiss against S. Typhimurium ATCC 14028. The result of Cohran test showed the growth of M. tuberculosis H37RV could obstructed with modification of LJ extra koumiss stored for 4, 6, and 8 days. The conclusion of this research that koumiss was effective to against pathogenic bacterias such as S. Typhimurium ATCC 14028 and M. tuberculosis H37RV.