Aquatic Product Technology

Extraction and Enzymatic Hydrolysis of Spirulina Protein as Antioxidant

Sun, 01 Jan 2023 00:00:00 GMT

Extraction and Enzymatic Hydrolysis of Spirulina Protein as Antioxidant Hardiningtyas, Safrina Dyah; Syuhada, Firdha Shafira; Setyaningsih, Iriani Spirulina sp. is a microalgae species that is consumed as a dietary supplement due to its high nutrition content, especially protein and its bioactivity as a radical scavenger. Extraction and hydrolysis processes are expected to enhance Spirulina’s functional characteristic and its bioactivity to inhibit free radicals 1,1-diphenyl-2-picrylhydrazyl (DPPH). The aim of this study was to compare the effect of two different extraction methods on protein concentration, degree of hydrolysis, DPPH free radical inhibition, and amino acid profile of Spirulina sp. hydrolysate. Spirulina sp. were extracted using two different methods, physical and chemical methods. The crude protein extracts were analysed using Biuret method and hydrolysed using papain enzyme. The protein hydrolysate was finally run through some analysis such as hydrolysis degree, amino acid profile, and DPPH scavenging activity. The two different extraction methods effected on protein concentration and degree of hydrolysis significantly (p < 0,05). Protein concentration of Spirulina sp. that were extracted using physical method is 67,4 ± 14,35 mg/mL, while Spirulina sp. that were extracted using chemical method is 53,41 ± 3,90 mg/mL. Hydrolysis degree of protein hydrolysate extracted using physical method is 77,141 ± 11,945% while Spirulina sp. that were extracted using chemical method is 35,081 ± 15,884%. Both Spirulina sp. extract and hydrolysate %inhibition decreased after storage. Hydrolysate extracted using physical method contains 8.711,56 mg/kg of amino acid, while hydrolysate extracted using chemical method contains 3.391,56 mg/kg.

Komposisi Kimia Mikroalga Laut Chaetoceros gracilis

Sun, 01 Jan 2023 00:00:00 GMT

Komposisi Kimia Mikroalga Laut Chaetoceros gracilis Setyaningsih, Iriani; Desniar; Ermayanti, Ely Many factors affect the growth and chemical composition of Chaetoceros gracilis, including media and cultivation conditions. Growth media used for cultivation of C. gracilis in this study was NPSi media. Cultivation of C. gracilis conducted outdoor. This study aimed to determine fatty acids profile and the chemical components of C. gracilis. Chaetoceros gracilis contained 25,56% SAFA, 34,77% MUFA, 39,55% PUFA. C. gracilis dry biomass contained of 27% water, 25% ash, 12,1% fat, 20,27% protein and 15.63% carbohydrate. Active components contained in the dry biomass were alkaloids, terpenoids, carbohydrates, reducing sugars, and amino acids. Calsium was the highest mineral contained in C. gracilis.

Kajian Produksi Biolistrik Dari Limbah Industri Hasil Perikanan Dengan Sistem Microbial Fuel Cell

Sat, 01 Jan 2022 00:00:00 GMT

Kajian Produksi Biolistrik Dari Limbah Industri Hasil Perikanan Dengan Sistem Microbial Fuel Cell Ibrahim, Bustami Microbial fuel cell (MFC) merupakan suatu teknologi alternatif penghasil energi listrik dari limbah cair. Industri pengolahan produk perikanan menghasilkan limbah cair yang tinggi dengan kandungan bahan organik yang tinggi, yaitu protein dan lemak. Banyak substrat terlibat dalam menghasilkan listrik termasuk karbohidrat, protein, asam volatil, selulosa yang digunakan sebagai umpan nutrisi dalam MFC yang dapat dibandingkan hasilnya dengan limbah cair perikanan. Konstruksi dan analisis MFC membutuhkan pengetahuan di bidang ilmiah dan rekayasa, mulai dari mikrobiologi dan elektrokimia hingga material yang digunakan. Dari hasil kajian disimpulkan bahwa untuk pengembangan lebih lanjut dari teknologi MFC menggunakan substrat limbah cair perikanan bahwa proses berjalan lebih lambat, potensi jenis mikroba yang berperan lebih luas, faktor keterbatasan dan desain konstruksi dalam sistem MFC masih perlu diteliti, sehingga akan hemat biaya dan untuk meningkatkan produksi listrik.

Pembuatan Pepton secara Enzimatis Menggunakan Bahan Baku Jeroan Ikan Tongkol

Mon, 01 Apr 2013 00:00:00 GMT

Pembuatan Pepton secara Enzimatis Menggunakan Bahan Baku Jeroan Ikan Tongkol Nurhayati, Tati; Desniar; Suhandana, Made