http://repository.ipb.ac.id/handle/123456789/29124 2026-04-12T08:49:46Z http://repository.ipb.ac.id/handle/123456789/87554 INSECTICIDAL ACTIVITY OF ANNONACEOUS SEED EXTRACTS AGAINST Crocidolomia binotalis ZELLER (LEPIDOPTERA: PYRALIDAE) Prijono, Djoko; Gani, Mahir S.; Syahputra, Edy Insecticidal Activity of Annonaceous Seed Extracts against Crocidolomia binotalis Zeller (Lepidoptera: Pyralidae) . Insecticidal activity of seed extracts of II species of Annonaceae was evaluated against Crocidolomia binotatis Zeller in a leaf-residue feeding bioassay. Acetone seed extracts of Annona glabra and A. squamosa showed strong insecticidal activity against C. binotatis and both extracts were more active than Derris elliptica root extract. Aqueous seed extracts of the two Annona species were also active against the test insect. Acetone extracts of three other Annona species (A. montana, A. reticulata and A. muricata) at 0.25% exerted only low to moderate lethal effect, whereas those of the other six species were inactive. The treatment with extracts of the latter three Annona species delayed pupation of C. binotatis larvae by only 0.20 - 0.51 day, whereas the delay caused by D. elliptica root extract was 1.42 days. Effect of A. glabra and A. squamosa extracts on development of C. binotatis larvae could not be determined since all test larvae had died at day 2. 1997-01-01T00:00:00Z http://repository.ipb.ac.id/handle/123456789/87553 INSECTICIDAL ACTIVITY OF MELIACEOUS SEED EXTRACTS AGAINST Crocidolomia binotalis ZELLER (LEPIDOPTERA: PYRALIDAE) Prijono, Djoko Acetone seed extracts of 21 species of Meliaceae wel:e evaluated for their insecticidal activity against second-instar larvae of Crocidolomia binotalis Zeller by a leaf-residue feeding method. The larvae were fed extract-treated broccoli leaves for 2 days. then were maintained on untreated leaves until the fourth-ins tar stage. Records were kept in regard to the area of leaves eaten during the 2-day feeding treatment, daily larval mortality, and developmental time of surviving larvae from instar II to ins tar IV. The seed extracts of Aglaia harmsiana, Azadirachta indica (neem) and Dysoxylum mollissimum at a concentration of 0.25% exhibited a strong insecticidal activity against C. binotalis larvae (95-100% mortality), those of Aglaia odoratissima and Trichilia trijuga showed a fairly strong activity (78% and 87% mortality), whereas the activity of other extracts varied from inactive to moderately active (0 - 73% mortality). The active extracts generally exhibited the three kinds of effects, i. e. antijeedant, insecticidal and developmental derangement, at similar levels. The active extracts at 0.25% inhibited feeding by C. binotalis larvae on treated leaves by 78.4 - 96.6% and prolonged the developmental time from instar II to instar IV by 2.2 - 4.2 days as compared with controls. Further studies are needed to identifY insecticidal compounds in the said active extracts, except neem which has been exhaustively studied. 1998-01-01T00:00:00Z http://repository.ipb.ac.id/handle/123456789/87542 EVALUATION OF INSECTICIDAL ACTIVITY OF MELIACEOUS PLANT EXTRACTS AGAINST Crocidolomia binotalis ZELLER (LEPIDOPTERA: PYRALIDAE) Lina, Eka Candra; Prijono, Djoko Insecticidal activity of leaf, twig, stem bark and/or seed extracts of seven species of Meliaceae was evaluated against second-instar larvae of Croci dolomia binotalis using lea/residual method. Ethyl acetate soluble fraction of methanolic extract of each plant material was used ill tAts stud) . The feeding treatment for 2 days with seed extracts of Aglaia elliptica, A. hannsiana, A. odoratissima and Iridrilia trijuga at 0.25% caused 98.3, 100, 100 and 91.5% larval mortality, respectively. A. harmsiana ig extract was somewhat active (72.9% larval mortality), Dysoxylum mollissimum bark extract was moderately active (500/0 mortality), T. trijuga leaf and twig extracts were inactive (0% mortality), while the other extracts (A. aspera barks, A. hannsiana leaves, and leaves and twigs of A. elliptica, A. odorata and A. odoratissima) were only weakly active (1.7-28% mortality). Based on mortality of the second and third ins tar, LCso of A. elliptica, A. hannsiana and A. odoratissima seed extracts against C. binotalis larvae were 0.11, 0.03 and 0.04%, respectively. In addition to lethal effect, the active extracts also prolonged the developmental time of the surviving larvae from the second to f ourth instar. For the same plant species, the length of developmental delay caused by extracts of different parts corresponded with the degree of their lethal effect. 1999-01-01T00:00:00Z http://repository.ipb.ac.id/handle/123456789/52976 Pemanfaatan Virus Simbion Dari Beberapa Spesies Parasitoid Untuk Meningkatkan Kinerja Eriborus Argenteopilosus (Cameron) Ratna, Endang Sri Polydnaviruses (PDV) are present in the female reproductive tissues of parasitoid Ichneumonids and Braconids. PDV symbionts were extracted from the ovaries of the parasitoid Apanteles spp. and host larval tissues of Spodoptera litura (Fabricius) attacked by parasitoid Snellenius manilae Ashmead and identified using the negative staining methods. The ultrasructure appearance of PDV derived from the calyx of parasitoid Diadegma semiclausum Helen (Family: Ichneumonidae) tissues are determined by the presence of nucleocapsid elliptical surrounded by double bilayer membranes. PDV derived from parasitoid S manilae (Family: Braconidae) have nucleocapsid that are cylindrical, individually or in groups surrounded by single bilayer membranes. The extraction and centrifugation of ovary parasitoid and infected host larval tissues by parasitoid obtained through the sucrose gradient procedure produced PDV sediment originated from four spesies such as Eriborus argenteopilosus (Cameron) and D. semiclausum (Family: Ichneumonidae) as well as S. manilae and Apanteles spp. (Family: Braconidae). Immunological methods that followed showed that each antigens (Ag PDV) are compatible against their antibodies (Abs) were produced. 2008-01-01T00:00:00Z