http://repository.ipb.ac.id/handle/123456789/26695 2026-04-07T13:15:38Z http://repository.ipb.ac.id/handle/123456789/103604 The using of participatory epidemiology (EP) approach in supporting of rabies control program in district of Karangasem, Bali, Indonesia Basri C.; Suandy I.; Mariner J.; Hendrickx S. The effort to control rabies in Bali requires an alternative approach to surveillance system that is cheaper, more effective and was validated such as participatory epidemiology (PE) technique. PE study was conducted to analyze the incidence of rabies and other things associated with the event using the PE. The study was conducted in 12 villages in four subdistricts randomly selected in District of Karangasem, Bali province. Data were collected by a group discussion using the techniques developed in PE as simple ranking, proportional piling, matrix scoring, seasonal calendar and mapping. The results of this study showed in the District of Karangasem found various animals that could transmit rabies as dogs, cats, bats, squirrels and monkeys. Dogs are non-livestock animals most commonly found in the environment and the most important role in people's lives. About 20% of all dogs in the area are estimated to have the disease, including rabies problem. The problems of rabies in dogs in the region are expected to have morbidity of 3.6 %, mortality of 3% and case fatality of 81.8%. In general, the incidences of dog bites on humans have increased, especially in the months of April and June. The pattern of increased incidence of bites do not occur simultaneously with an increase in the number of breeding dogs at breeding season (February-April) and increased number of cultural and religious ceremonies (April and August). Our study revealed, that PE is a useful approach in an effort to control non-livestock animal diseases such as rabies. Based on our experiance, PE could be a useful tool to evaluate the success of public awareness program concerning rabies in Bali. 2012-01-01T00:00:00Z http://repository.ipb.ac.id/handle/123456789/76483 Purifikasi Dan Pencirian Enzim Protease Fibrinolitik Dari Ekstrak Jamur Merang Sajuthi, Dondin; Suparto, Irma; Yanti; Praira, Willy Purification and Characterization of Fibrinolytic Proteases from Mushroom Volvariela volvaceae Extract. Edible straw mushroom (V. volvaceae) has been known used for improvement of blood circulation due to its fibrinolytic content. The objective of the study is to purify and characterize fibrinolytic protease from straw mushroom extract. Purification were performed through several steps, i.e. precipitation using ammonium sulphate 75%, dialyzed membran (cut-off 10 kDa), and ion-exchange chromatography using DEAE Sepharose. The active fraction of DEAE-Sepharose contains two purified protein bands with molecular weight of 12.9 and 15.8 kDa. The active fraction has specific activity of 0.383 U/mg with 2.7 fold higher purification compared to its crude extract. Both crude and purified enzymes had optimum activity at temperature of 50 ºC and pH 7 in 10 minutes of incubation. Fibrin zymographic profile demonstrated that the enzyme hydrolyzed fibrin, as well as casein, indicating their potent fibrinolytic activity. The enzyme was strongly inhibited by phenilmethylsulphonyl fluoride and N-p-tosil-L-lysinchloromethyl keton. This suggested that it was a serine protease. In summary, these results showed that crude and purified protease of straw mushroom (V. volvaceae) has fibrinolytic activities that can be applied for alternative thrombolytic therapy. 2010-01-01T00:00:00Z http://repository.ipb.ac.id/handle/123456789/76450 Cyclo (Tyrosyl-Prolyl) Produced by sp.: Bioactivity and Molecular Structure Elucidation Streptomyces Sunaryanto, Rofiq; Marwoto, Bambang; Hartoto, Liesbetini; Mas'ud, Zainal Alim; Irawadi, Tun Tedja Determination of bioactivity by minimum inhibitory concentration (MIC) methods and molecular structure identification of antibiotic produced by sp. have been carried out. The antibiotic was produced by liquid culture using sp. isolate. Purification of antibiotic was carried out by silica gel column chromatography and preparative HPLC. Molecular structure identification was carried out using ESI-MS, H NMR, C NMR, and C DEPT NMR. Pure antibiotic showed inhibition activity to Gram-negative and Gram-positive bacteria. MIC to ATCC 25922, ATCC27853 , ATCC25923, and ATCC66923 were 27.0, 68.7, 80.2, and 73.7 μg mL , respectively. Identification using ESI-MS showed that the molecular weight of this antibiotic was 260 g mol , and molecular formula was C H N O . Elucidation of molecular structure using HNMR, C NMR, and C DEPTNMRshowed that antibiotic was cyclo(tyrosyl-prolyl). 2011-01-01T00:00:00Z http://repository.ipb.ac.id/handle/123456789/60973 Trichophyton mentagrophytes sebagai agen penyebab dermatofitosis pada kambing Sunartatie, Titiek Causative agent of dermatophytosis in goats and sheep is usually Trichophyton, the most common is Trichophyton verrucosum. The aim of this study was to isolate and identify causative agent of dermatophytosis in the Peranakan Etawah (PE) goats. Scrapped skin from the PE goats, that was presumed suffering dermatophytosis was collected as a sample. By direct microscopic examination of the sample, septat hypha and macroconidia with shape that characterize the Trichophyton mold were found. The result of sample isolation on the Dermasel agar showed that the mold colony grow after 5 days. Based on macroscopic and microscopic morphology study, the mold was identified as T. mer.tagrophytes. 2010-01-01T00:00:00Z