http://repository.ipb.ac.id/handle/123456789/39 2026-04-11T22:13:00Z 2026-04-11T22:13:00Z Ambarsari, Laksmi Suryani Gozales, Steffanus Puspita, Puspa Julistia http://repository.ipb.ac.id/handle/123456789/76076 2015-09-02T00:28:50Z 2015-01-01T00:00:00Z

The Addition Effects of Glucose as a Co-substrate on Xylitol Production by Candida guilliermondii Ambarsari, Laksmi; Suryani; Gozales, Steffanus; Puspita, Puspa Julistia ·High cost production is one of the constraints of the commercial xylitol production due to high energy needed and pure raw materials. Therefore, it is necessary to improve the xylitol production eficiently with lower production cost by using microorganisms. The research objectives were to determine the optimum xylitol productionfi'om xylose by metabolism of C. guilliermondii and effect of glucose as a co-substrate in fermentation medium. The ratio of glucose: xylose (g/L) was 1 :25, 1: 12, 1 :5 and 1 :2.5 respective(v. The xylitol concentration was measured by spectrophotometer method (Dsorbytol! D-xylitol kit). The result showed that the exponential phase of Candida guilliermondii was 12 h to 36 of incubation and optimum of incubation time to produce the highest xylitol was 72 h. The best ratio- of glucose : xylose to produce xylitol was 9 g/L glucose : 45 g!L xylose (1 : 5). The xylitol concentration produced fi'om medium with the addition of glucose was 2.85 g!L. This concentration increased five times compared to that in the medium without addition of glucose that only reached 2.85 g/L. According to this study, the addition of glucose as a co-substrate could increase the xylitol production.

2015-01-01T00:00:00Z Cing, Jap Mai Seno, Djarot Sasongko Hami Santoso, Tri Joko http://repository.ipb.ac.id/handle/123456789/76075 2015-09-01T22:40:05Z 2015-01-01T00:00:00Z

Identification of Aroma Gene (Mutated badh2) and Properties of Aroma on Aromatic BC5F 2 Ciherang Cing, Jap Mai; Seno, Djarot Sasongko Hami; Santoso, Tri Joko Aromatic rice varieties have some weaknesses such as low productivity, and less resistant to pests and diseases. This study aimed to obtain homo:::ygous strain ofBCF'2 Ciherang aromatic through the identification of aroma gene (mutated badh2) and properties of the aroma. Ciherangpaddy (nonaromatic paddy) was used as the female parent, whereas Mentik Wangi paddy (aromatic paddy) was used as the male parent. The experiment was conducted in BCJ2 because it is expected to generate plants with properties 98. 4% close to female parent. The DNA from five strains of paddy plants BC J 2 Ciherang X }vfentik Wangi was isolated by a modified CTAB melhod. The concenlralion of DNA was determined by measuring absorbance at 260 nm wavelength, while its purity was determined from the ratio of the absorbance at a wavelength of2601280 nm. PCR-based molecular selection was done by using the Bradbury primers. PCR results showed that of the 250 samples, there were 66 samples had DNA.fragment of the same size as that of Mentik Wangi, i.e. 257 bp, 67 samples had the same size as the DNAfragment ofCiherang, i.e. 355 bp, and 117 samples had the same size with the both of DNA fragments, i.e. 257 bp and 355 bp. Plants with amplified 257 bp DNA fragment was subjected to leaf aroma test using 1. 7% KOH. The results showed that 42 positive samples, out of 66 samples. Samples positive on leaf aroma test were tested again on rice aroma test. Rice aroma test results showed the majority (85.4%) samples that are positive on leaf aroma lest is also positive on the rice aroma lesl.

2015-01-01T00:00:00Z Setyawati, Inda Ambarsari, Laksrni Nur'aeni, Siti Suryani Puspita, Puspa Julistia Kurniatin, Popi Asri Nurcholis, Waras http://repository.ipb.ac.id/handle/123456789/76074 2015-09-01T23:45:03Z 2015-01-01T00:00:00Z

Bioethanol Production by Using Detoxified Sugarcane Bagasse Hydrolysate and Adapted Culture of Candida tropicalis Setyawati, Inda; Ambarsari, Laksrni; Nur'aeni, Siti; Suryani; Puspita, Puspa Julistia; Kurniatin, Popi Asri; Nurcholis, Waras Ethanol is considered as the most promising alternative fuel, since it can be produced from a variety of agriculturally-based renewable materials, such as sugarcane bagasse. Lignocellulose as a major component of sugarcane bagasse is considered as an attractive renewable resource for ethanol production due to its great availability and relatively low cost. The major problem of lignocellulose is caused by its need/or treatment to be hydrolyzed to simple sugar before being used/or bioethanol production. However, pretreatment using acid as hydroZvzing agent creates some inhibitor compoundr; that reduce ethanol production because these compounds are potential fermentation inhibitors and qffect the growth rate of the yeast. Reduction of these by-products requires a conditioning (detoxification and culture starter adaptation). Thus, the aim of this study was to evaluate bioethanol production by fermentation with and without detoxified sugarcane bagasse acid hydrolysate using adapted and non-adapted culture of C. tropical is. According to this study, the highest ethanol amount was obtained about 0.43 % (vlv) with an ethanol yield of 2.51 % and theoretical yield of 4.92 % by fermentation of sugarcane bagasse hydrolysate with detoxification using the adapted strain of C. tropicalis at 72 hours fermentation time. Furthermore, the addition of 3 % glucose as co-substrate on detoxified-hydrolysate media only achieved the highest ethanol concentration 0.21 % after 24 hours fermentation with the ethanol yield 0.69 % and theoretical ethanol yield 1.35 %, thus it can be concluded that the addition of glucose could not increase the ethanol production.

2015-01-01T00:00:00Z Hasan, Akhmad Endang Zainal Hawab, Mansyur Bintang, Maria http://repository.ipb.ac.id/handle/123456789/65661 2013-10-09T02:47:09Z 2013-10-09T02:47:09Z

Pengaruh Zat Tumbuh terhadap Perkembangan Tunas Artemisia annua Hasan, Akhmad Endang Zainal; Hawab, Mansyur; Bintang, Maria Lima tipe dan Artemisia annua telah ditanam pada media padat yang mengandung zat tumbuh(BAP, Kinetin, IAA dan NAA). Artemisia annua ini dapat menghasilkan artemisin yang efektif mengobati malaria, juga dapat dipakai sebagai anti bakteri dan bahkan sebagai anti kanker. Oari lima tipe yang dicobakan hanya tipe D yang sangat baik pertumbuhan tunasnya, baik tinggi tunas maupun jumlah tunas samping dibanding dengan yang lain dari media yang dicobakan. Nusa Kimia (jurnal Ilmu-Ilmu Kimia), Vol.3, No.1, Juni 2003

2013-10-09T02:47:09Z