Browsing by Author "Madayanti, Fida"
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Cell Lysis Method Affects Assessment of Microbial Diversity Based on Ribotyping Analysis
Yohandini, Heni | Madayanti, Fida | Aditiawati, Pingkan | Akhmaloka (2008)The microbial community in Kawah Hujan, Kamojang, West Java, Indonesia, was analyzed using 16S-rRNA-gene-sequencing combining with denaturing-gradient-gel electrophoresis (DGGE) technique. Two different cell lysis methods, ... -
Cloning, Homological Analysis, and Expression of DNA Pol I from Geobacillus thermoleovorans
Akhmaloka | Pramono, Hendro | Ambarsari, Laksmi | Susanti, Evi | Nurbaiti, Santi | Madayanti, Fida (2008)DNA POLI gene from Geobacillus thermoleovorans (DNA POLI ITB) was cloned and overexpressed in Escherichia coli using two steps PCR aplification. The gene was isolated and sequenced to consist of 2628 nucleotides that encode ... -
The Effect of Mutation at Thr 295 of Saccharomyces cerevisiae eRF1 on Suppression of Nonsense Codons and eRF1 Structur
Susilowati, Prima Endang | Aditiawati, Pingkan | Madayanti, Fida | Akhmaloka (2008)The termination of translation in Saccharomyces cerevisiae is controlled by two interacting polypeptide chain release factors, eRF1, and eRF3. Two regions in eRF1, at position 281-305 and 411-415, were proposed to be ... -
Sixteen-S Ribosomal RNA-Based Analysis of Thermophilic Bacteria in Gedongsongo Hot Spring
Aminin, Agustina Lulustya Ningati Nurul | Madayanti, Fida | Aditiawati, Pingkan | Akhmaloka (2007)Denaturing gradient gel electrophoresis was used to identify the bacterial community at the Gedongsongo (WGS-2) hot spring. The bacterial samples were obtained from both culture dependent and independent strategies. Partial ...