| dc.description.abstract | Enterocytozoon hepatopenaei (EHP) is a type of parasite that can be
categorized as a microsporidia, which is a diverse group of obligate intracellular
eukaryotic parasites. EHP infection is often associated with white feces syndrome. The most of the
aquaculture shrimp contribution has come from Penaeus vannamei which accounts 80% of the whole
shrimp production. The aim of this research is to analyze and determine the structures and
intensity of these organisms through microscopic observation of the Penaeus vannamei shrimp that
were confirmed to be infected by Enterocytozoon hepatopenaei through polymerase chain reaction
(PCR). This research is done in the form of histopathological observations through diagrams, and
the understanding of the pathogenesis using Hematoxylin Eosin (HE), Alcian Blue-Periodic Acid
Schiff (AB-PAS) and Masson’s Trichrome staining. Histopathological representation is done
through the use of a light microscope. Results of this study indicates that, the testing of three
types of dyes which are the Hematoxylin Eosin (HE) stain, Alcian-Blue Periodic Acid Schiff (AB-PAS)
and the Masson’s Trichrome stain show that the usage of AB-PAS stain is the best out of the three
stains. Further tests show that granulocytes are the type of hemocytes most dominant in response to
EHP infection with the highest susceptibility in shrimp 1 out of three shrimp. Intestinal
microscopic observation also showed that there was no apparent occurrence of white feces syndrome
(WFS). In conclusion, from this research it can be found microscopically an abnormality which is
the presence of one
of the life cycles of the parasite EHP in the hapatopancreas tubules of the shrimp. | id |
